自然杀伤细胞免疫疗法的创新策略及研究进展  

Innovation Strategies and Research Progress of Nature Killer Cell Immunotherapy

作　　者：张华 杜为 邱录贵[5,6,7] 张宇 ZHANG Hua;DU Wei;QIU Lugui;ZHANG Yu(Union Stem Cell&Gene Engineering Co.,Ltd,Tianjin 300384,China;School of Medicine,Nankai University,Tianjin 300071,China;Tianjin Municipal Key Laboratory of Blood Cell Therapy Technology,Tianjin 300384,China;State Industrialization Base for Stem Cell Engineering Products,Tianjin 300384,China;Tianjin Municipal Bank of Cord Blood Hematopoietic Stem Cell,Tianjin 300384,China;Institute of Hematology&Blood Diseases,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300020,China;State Key Laboratory of Experimental Hematology,Tianjin 300020,China;Vcanbio Cell&Gene Engineering Co.,Ltd,Tianjin 300384,China)

机构地区：[1]协和干细胞基因工程有限公司,天津300384 [2]南开大学医学院,天津300071 [3]天津市血液细胞治疗技术企业重点实验室,天津300384 [4]国家干细胞工程产品产业化基地,天津300384 [5]天津市脐带血造血干细胞库,天津300384 [6]中国医学科学院北京协和医学院血液学研究所血液病医院,天津300020 [7]实验血液学国家重点实验室,天津300020 [8]中源协和细胞基因工程股份有限公司,天津300384

出　　处：《药学进展》2023年第1期43-55,共13页Progress in Pharmaceutical Sciences

基　　金：国家重点研发计划(No.2022YFA1105600);武汉市科技计划项目(No.2020020602012112);细胞生态海河实验室创新基金(No.HH22KYZX0046);天津自贸试验区创新发展项目(No.ZMCY-03-2021002-01)。

摘　　要：自然杀伤(NK)细胞是一种天然的免疫淋巴细胞,能够对病毒感染的和恶性转化的细胞产生有效的免疫应答。鉴于其独特的识别杀伤机制,NK细胞在肿瘤免疫治疗领域受到了广泛关注。然而,NK细胞单一疗法的体内持久性和有效性面临巨大挑战。为了解决这个难题,越来越多的研究聚焦于NK细胞过继转移治疗策略的开发。通过对NK细胞基础生物学知识进行综述,并且总结不同来源的治疗性NK细胞的特征以及临床级NK细胞的制备方法,对不断发展的靶向NK细胞功能反应的基因操控和过继转移治疗策略进行概述,为NK细胞免疫治疗产品的开发提供参考。Natural killer(NK)cells,as natural immune lymphocytes,mediate an effective immune response towards virus-infected and malignant transformed cells.Due to its specific recognition and killing mechanism,NK cell has gained wide attention in the field of cancer immunotherapy.However,the durability and effectiveness of NK cell monotherapy in vivo face great challenges.To overcome this problem,more and more studies now focus on the development of therapeutic strategies for NK cell adoptive transfer.This paper reviews the basic biology of NK cells,summarizes the characteristics of different sources of therapeutic NK cells and the methods for the generation of clinical-grade NK cells,and outlines the evolving strategies for genetic manipulation and adoptive transfer that target the functional response of NK cells,so as to provide some reference for the development of NK cell immunotherapy products.

关 键 词：自然杀伤细胞 肿瘤 免疫疗法 细胞产品 治疗策略 

分 类 号：R730.51[医药卫生—肿瘤]