适配体生物传感器在黄曲霉毒素B1检测中的应用  

Application of Aptamer Biosensor in Detection of Aflatoxin B1

作　　者：韩文浩 李延生 高国伟[1,2] 张学记 HAN Wen-hao;LI Yan-sheng;GAO Guo-wei;ZHANG Xue-ji(Key Laboratory of Sensors,Beijing Information Science and Technology University,Beijing 100101,China;Key Laboratory of Modern Measurement and Control Technology of Ministry of Education,Beijing Information Science and Technology University,Beijing 100192,China;School of Biomedical Engineering,Shenzhen University,Shenzhen 518060,China)

机构地区：[1]北京信息科技大学传感器重点实验室,北京100101 [2]北京信息科技大学现代测控技术教育部重点实验室,北京100192 [3]深圳大学生物医学工程学院,广东深圳518060

出　　处：《分析测试学报》2023年第3期368-374,共7页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(62101053);北京市教育委员会科学研究计划项目(KM202211232004)。

摘　　要：该文首先对黄曲霉毒素B1(AFB1)的相关性质及其传统检测方法进行了介绍,随后概述了近年来基于光学、电化学以及微流控芯片的适配体生物传感器的构建及其在AFB1检测领域中的应用,旨为适配体生物传感器的实际应用提供参考;并通过探讨目前开发的检测方法存在的问题,对适配体生物传感器前景和未来趋势进行了展望。Aflatoxin B1(AFB1)has strong toxicity and carcinogenicity,and its dose exceeding the standard will seriously endanger the health of organisms.The development of new AFB1 detection methods is of great significance for the prevention and control of its harm.The traditional method for AFB1 detection is mature and reliable,with low detection limit,but relying on professional equipment and professionals.It cannot meet the needs of on-site detection.Aptamer biosensors have the advantages of high sensitivity,high specificity and simple operation,which have attracted attention in the detection of AFB1.Meanwhile,researchers have developed many new methods based on different principles.The related properties of AFB1 and its traditional detection methods are firstly introduced in this paper,and then the construction of aptamer biosensors based on optical,electrochemical and microfluidic chips in recent years as well as their applications in the field of AFB1 detection are summarized.It provides a reference for the development of practical application of aptamer biosensors,and discusses the development prospects and future trends of aptamer biosensors by discussing the problems in the currently developed detection methods.

关 键 词：黄曲霉毒素B1 适配体 生物传感器 应用 

分 类 号：O657[理学—分析化学] S951.4[理学—化学]