YC-1增强β-榄香烯的致人骨肉瘤细胞凋亡作用  

Enhancing Effect of YC-1 on Saos-2 Cells Apoptosis Induced by β-elemene

作　　者：梁单 李朋举 郭琳[2] 刘胜权[1] 刘司琪[1] LIANG Dan;LI Pengju;GUO Lin;LIU Shengquan;LIU Siqi(Troops of 95988 Unit,Changchun 130000,China;Troops of 93253 Unit,Dalian 116000,China)

机构地区：[1]中国人民解放军95988部队,吉林长春130000 [2]中国人民解放军93253部队,辽宁大连116000

出　　处：《湖北民族大学学报（医学版）》2023年第1期6-8,13,共4页Journal of Hubei Minzu University(Medical Edition)

摘　　要：目的 探讨YC-1对β-榄香烯诱导人骨肉瘤细胞(Saos-2)凋亡中的增强作用及作用机理。方法 Saos-2贴壁培养24,48和72 h后,MTT方法检测细胞增殖情况。贴壁培养48 h后,Western-blot方法,流式细胞术等方法检测Saos-2凋亡。Western-blot实验方法测定凋亡相关蛋白及HIF-1α蛋白水平。结果 β-榄香烯可以诱导细胞凋亡,增加HIF-1α蛋白的表达水平,而HIF-1α抑制剂YC-1能加强β-榄香烯的致凋亡作用。结论 YC-1通过上调HIF-1α蛋白表达,增加β-榄香烯诱导的Saos-2凋亡。Objective To investigate the enhancing effect of YC-1 on Saos-2 cells apoptosis induced by β-elemene and its mechanism.Methods Cell proliferation was detected by MTT after 24,48 and 72 hours of culture.The apoptosis of Saos-2 cells was detected by Western blot and flow cytometry after 48 hours of adherent culture.The apoptosis related proteins and HIF-1 were detected by western blot.Result β-elemene can induce apoptosis and increase HIF-1α Protein level.YC-1(HIF-1α inhibitor) can increase the effect of apoptosis induced by β-elemene.Conclusion Saos-2 cells apoptosis induced by β-elemene can be enhanced by YC-1 through upregulation HIF-1α protein.

关 键 词：YC-1 Β-榄香烯 凋亡 HIF-1Α 人骨肉瘤细胞 

分 类 号：R738.1[医药卫生—肿瘤]