黄芪甲苷对结直肠癌HCT116细胞增殖、迁移及侵袭的影响  

Effect of Astragaloside Ⅳ on Proliferation, Migration, and Invasion of Colorectal Cancer HCT116 Cells

作　　者：侯本超 何志坚[2] 刘海云[3] 林倩霞 方永青 占诗梦 HOU Benchao;HE Zhijian;LIU Haiyun;LIN Qianxia;FANG Yongqing;ZHAN Shimeng(The First Affiliated Hospital of Nanchang University,Nanchang 330006,China;Jiangxi Cancer Hospital,Nanchang 330029,China;School of Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]南昌大学第一附属医院,南昌330006 [2]江西省肿瘤医院,南昌330029 [3]江西中医药大学中医学院,南昌330004

出　　处：《中国实验方剂学杂志》2023年第5期144-149,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江西省教育厅科学技术研究项目(200143,GJJ201210);江西省大学生创新创业项目(s202010412060,s202010412077);CSCO-恒瑞肿瘤研究基金项目(Y-HR2017-128);江西省卫生厅中医药课题项目(2019B085)。

摘　　要：目的:探究黄芪甲苷对结直肠癌HCT116细胞增殖、迁移及侵袭能力的影响,并探讨其相关分子机制。方法:将结直肠癌HCT116细胞分为空白组(DMSO)和黄芪甲苷低、中、高质量浓度组(15.7、31.4、62.8 mg·L^(-1))。通过倒置显微镜观察给药后结直肠癌HCT116细胞形态的改变;细胞增殖与活性检测-8(CCK-8)法检测黄芪甲苷处理后细胞活性的改变;细胞划痕实验和Transwell实验观察黄芪甲苷处理后结肠癌HCT116细胞迁移及侵袭能力的变化;蛋白免疫印迹法(Western blot)检测结直肠癌HCT16细胞周期蛋白依靠性激酶抑制剂(p21)、细胞周期蛋白D_(1)(CyclinD_(1))及B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)表达水平。结果:与空白组比较,黄芪甲苷低、中、高质量浓度组细胞生长速度慢,密度明显稀疏,细胞形态不一,细胞核逐渐缩小,细胞质逐渐降解,细胞死亡率高,细胞活力呈浓度依赖性下降,细胞迁移和侵袭能力明显受到抑制(P<0.05,P<0.01),其抑制率与给药浓度呈正相关;与空白组比较,黄芪甲苷低、中、高质量浓度组促凋亡蛋白Bax表达量逐渐升高,呈浓度依赖性,细胞周期蛋白p21、CyclinD_(1)及抑制凋亡蛋白Bcl-2表达量逐渐降低(P<0.05,P<0.01),呈浓度依赖性。结论:黄芪甲苷可抑制结直肠癌HCT116细胞的增殖、迁移和侵袭能力,促进细胞凋亡,从而抑制结直肠癌的发生发展。Objective:To investigate effect of astragaloside Ⅳ on the proliferation, migration, and invasion of colorectal cancer HCT116 cells and the underlying molecular mechanism. Method:Colorectal cancer HCT116 cells were classified into blank group(DMSO) and low-dose(15.7 mg·L^(-1)), medium-dose(31.4 mg·L^(-1)), and high-dose(62.8 mg·L^(-1)) astragaloside Ⅳ groups. After drug treatment, the morphological changes of HCT116 cells were observed under an inverted microscope. Cell viability was detected by cell counting kit-8(CCK-8) assay, and the migration and invasion of cells were detected based on scratch assay and Transwell assay. The expression of cyclin-dependent kinase inhibitor(p21), CyclinD_(1), B-cell lymphoma-2(Bcl-2), and Bcl-2-associated X protein(Bax) in the cells was examined by Western blot. Result:Compared with the blank group, cells in the three astragaloside Ⅳ groups demonstrated slow growth, low density, inconsistent morphology, nuclear shrinkage, degradation of cytoplasm, and high death rate. Moreover, cell viability decreased in a concentration-dependent manner in the astragaloside Ⅳ groups. Cell migration and invasion were inhibited(P<0.05, P<0.01), and the inhibition rate was in positive correlation with the concentration of the astragaloside Ⅳ. The expression of pro-apoptotic protein Bax in low-dose, medium-dose and high-dose astragaloside Ⅳ groups increased gradually in a concentration-dependent manner, while the expression of p21, CyclinD_(1)and anti-apoptotic protein Bcl-2 decreased gradually in a concentration-dependent manner compared with those in the blank group(P<0.05, P<0.01). Conclusion:Astragaloside Ⅳ can suppress the proliferation, migration, and invasion of colorectal cancer HCT116 cells and promote the apoptosis, thus inhibiting the occurrence and development of colorectal cancer.

关 键 词：黄芪甲苷 结直肠癌 增殖 迁移 侵袭 

分 类 号：R22[医药卫生—中医基础理论] R242[医药卫生—中医学] R2-031R285.5