超高速细胞分选平台结合cDNA microarray技术筛查宫颈癌细胞潜在分子标志物  

Screening of potential molecular markers in cervical cancer cells by ultra-high-speed cell selection platform combined with cDNA microarray technology

作　　者：陈红香[1] 周自华[2] 周艳宏 Chen Hongxiang;Zhou Zihua;Zhou Yanhong(Department of Gynecology,Xinjiang Uygur Municipal People's Hospital;Department of Oncology,Loudi Central Hospital of Hunan;Cancer Research Institute,School of Basic Medical Sciences,Central South University)

机构地区：[1]新疆维吾尔自治区人民医院妇科,乌鲁木齐830001 [2]湖南娄底市中心医院肿瘤科,长沙410078 [3]中南大学基础医学院肿瘤研究所,长沙410011

出　　处：《重庆医科大学学报》2023年第1期54-60,共7页Journal of Chongqing Medical University

基　　金：新疆维吾尔自治区自然科学基金资助项目(编号:2017D01C122)。

摘　　要：目的:通过超高速细胞分选平台结合cDNA microarray技术,筛查宫颈癌细胞可能潜在的分子标志物。方法:采用MoFlo XDP型超高速细胞分选平台纯化细胞膜表面表达CD38和不表达CD38的宫颈癌细胞,利用RNAlater技术得到cDNA microarray实验所需RNA,然后进行基因芯片分析。结果:利用MoFlo XDP型超高速细胞分选平台可以获得纯度为99.0%以上的CD38阳性表达宫颈癌细胞。结论:cDNA microarray分析发现了RORA、PLIN4、AUTS2、IFITM1等宫颈癌细胞潜在分子标志物,为宫颈癌研究提供了新的技术方法。Objective:To screen potential molecular markers of cervical cancer cells by ultra-high speed cell sorting platform combined with cDNA microarray technology. Methods:In this paper,the MoFlo XDP-type ultra-high-speed cell selection platform was used to purify cervical cancer cells expressing CD38 and not expressing CD38 on the cell membrane surface. RNAlater technology was used to obtain the RNA required for cDNA microarray experiments,and then by gene chip analysis was made. Results:The MoFlo XDP-type ultra-high-speed cell selection platform can be used to obtain cervical cancer cells with a CD38-positive expression of 99.0% purity. Conclusion:Through the analysis of cDNA microarray,potential molecular markers of cervical cancer cells such as RORA,PLIN4,AUTS2,and IFITM1 have been found,which provides a new technical method for the research of cervical cancer.

关 键 词：流式细胞术 cDNA microarray 宫颈癌 分子标志物 

分 类 号：R737.3[医药卫生—肿瘤]