PIPS联合纳米银对根管粪肠球菌抗菌效果研究  

Efficacy of the photon-induced photoacoustic streaming combined with nano-silver solution on Enterococcus faecalis in root canals

作　　者：崔静雯 范建林[1] CUI Jingwen;FAN Jianlin(Department of Siomatology,the Second Afiliated Hospial of Soochow University,Suzhou 215000,China)

机构地区：[1]苏州大学附属第二医院口腔科,江苏苏州215000

出　　处：《口腔医学》2023年第2期110-113,共4页Stomatology

基　　金：国家自然科学基金预研项目(SDFEYGJ2007)。

摘　　要：目的 探究激光引发光声流系统(PIPS)和纳米银(AgNPs)联合使用对根管内粪肠球菌生物膜的抗菌效果。方法 收集36颗单根管离体牙,建立粪肠球菌感染根管实验模型,将样本随机分成6组,采用0.9%NaCl、2%NaClO、0.1%AgNPs溶液分别联合传统手动冲洗(CNI)或PIPS对根管进行冲洗,使用菌落计数法测定治疗前后根管内粪肠球菌生物膜菌落数,并计算菌落计数减少的百分比。结果 所有实验组粪肠球菌生物膜的抑制效果均强于对照组(P<0.05),使用PIPS辅助0.9%NaCl、2%NaClO、0.1%AgNPs冲洗组的降幅均分别大于CNI辅助0.9%NaCl、2%NaClO、0.1%AgNPs冲洗组(P<0.05)。PIPS辅助0.1%AgNPs冲洗组的降幅明显大于PIPS辅助2%NaClO冲洗组(P<0.05)。结论 PIPS辅助AgNPs溶液冲洗可以显著提高根管内粪肠球菌生物膜的清除效果。Objective To explore the antibacterial effect of combined use of photon-induced photoacoustic streaming(PIPS) and silver nanoparticles(AgNPs) on Enterococcus faecalis biofilm in root canals. Methods A total of 36 isolated teeth with single root canal were collected to establish an experimental root canal model of Enterococcus faecalis infection. Samples were randomly divided into six groups and 0.9% NaCl, 2% NaClO, 0.1% AgNPs solutions were used with conventional needle irrigation(CNI) or PIPS for root canals. Colony count method was used to measure the number of Enterococcus faecalis biofilm in root canals before and after treatment, and the percentage of colony count reduction was calculated. Results The inhibitory effect of Enterococcus faecalis biofilm in all experimental groups was stronger than that in the control group(P<0.05). The decrease amplitude of 0.9% NaCl, 2% NaClO, 0.1% AgNPs assisted with PIPS was greater than that of 0.9% NaCl, 2% NaClO, 0.1% AgNPs assisted with CNI(P<0.05). The decrease in the 0.1% AgNPs assisted with PIPS group was significantly greater than that in the 2% NaClO assisted with PIPS group(P<0.05). Conclusion PIPS-assisted AgNPs solution washing can significantly improve the effect of clearing Enterococcus faecalis biofilm in root canals.

关 键 词：纳米银(AgNPs) 激光引发光声流系统(PIPS) 粪肠球菌 

分 类 号：R781.05[医药卫生—口腔医学]