马铃薯响应低温胁迫的蛋白质组学分析  

Proteomic Analysis of Potato Response to Low Temperature Stress

作　　者：李文翔 王舰[1,2,3,4,5] 王芳 LI Wenxiang;WANG Jian;WANG Fang(Academy of Agricultural and Forestry Sciences,Qinghai University,Xining 810016,China;Qinghai Academy of Agriculture and Forestry Sciences,Xining 810016,China;Key Laboratory of Qinghai-Tibetan Plateau Biotechnology of Ministry of Education/Key Laboratory of Potato Breeding of Qinghai,Xining 810016,China;Northwest Potato Engineering Research Center of Ministry of Education,Qinghai University,Xining 810016,China;State key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农林科学院,西宁810016 [2]青海省农林科学院,西宁810016 [3]青藏高原生物技术教育部重点实验室/青海省马铃薯育种重点实验室,西宁810016 [4]青海大学西北马铃薯教育部工程研究中心,西宁810016 [5]青海大学省部共建三江源生态与高原农牧业国家重点实验室,西宁810016

出　　处：《西北农业学报》2023年第4期551-565,共15页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：财政部和农业农村部:国家现代农业产业技术体系(CARS-10);青海省科技厅项目:马铃薯种薯微型化及标准化生产技术研究与示范(2020-NK-170);宁波市2025重大种业专项:优质特色薯类新品种选育及应用推广(2019B10017)。

摘　　要：为探究马铃薯在蛋白质组学水平上对低温胁迫的响应机制,对耐低温品种'DR-2'与低温敏感品种'费乌瑞它'组培苗叶片蛋白质组学进行分析.结果表明,鉴定到肽段282790个,唯一性肽段19099个,蛋白质4471个.通过Label-Free技术对叶片差异表达蛋白分析表明,低温胁迫下'DR-2'中有38个显著差异蛋白,'费乌瑞它'中有72个显著差异蛋白.筛选出代谢通路差异蛋白13个,包括代谢相关蛋白5个,光合作用相关蛋白3个,防御相关蛋白2个,运输相关蛋白3个.Pathway富集分析结果表明低温胁迫处理下,分布在光合作用、能量代谢通路中的3-磷酸甘油醛脱氢酶、钙网蛋白、热激蛋白、肌醇-3-磷酸合酶在叶片中差异表达,该结果对研究马铃薯低温响应机制具有一定参考价值.In order to explore the response mechanism of potato to low temperature stress at the pro-teomic level,the leaf proteomic of the plantlets of the low-temperature-tolerant variety'DR-2’and the low-temperature sensitive variety‘Favorite'were analyzed in this study,the results showed that there were 282790 peptides,19099 unique peptides and 4471 proteins,and there were 38 differentially ex-pressed proteins in‘DR-2’and 72 differentially expressed proteins in‘Favorite’under low tempera-ture stress by Label-Free technique.And 13 of them were screened outin metabolic pathways,inclu-ding 5 metabolism-related proteins,3 photosynthesis-related proteins.2 defense-related proteins and 3 transport-related proteins.Pathway enrichment analysis results showed that glyceraldehyde-3-phos-phate dehydrogenase,calreticulin.heat shock protein,and inositol 3-phosphate synthase distributed in photosynthesis and energy metabolism pathways were differentially expressed in leaves under low temperature stress.This study providesa reference for study on the low temperature response mecha-nism of potato.

关 键 词：马铃薯 低温胁迫 蛋白质组学 响应机制 

分 类 号：S532[农业科学—作物学]