高盐摄入对C57BL/6J小鼠肾脏损伤和纤维化的影响  

Effects of High Salt-intake on Renal Injury and Fibrosis in C57BL/6J Mice

作　　者：成钰莹 谭舒丹 陈燕兰 黄达荣 邓新宇 黄俊源 黄皓璋 黎攀 杜冰[1,2] CHENG Yuying;TAN Shudan;CHEN Yanlan;HUANG Darong;DENG Xinyu;HUANG Junyuan;HUANG Haozhang;LI Pan;DU Bing(College of Food,South China Agricultural University,Guangzhou 510642,China;Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,China;Guangdong AIB Polytechnic College,Guangzhou 510507,China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]岭南现代农业科学与技术广东省实验室,广东广州510642 [3]广东农工商职业技术学院,广东广州510507

出　　处：《食品工业科技》2023年第5期397-402,共6页Science and Technology of Food Industry

基　　金：“财政部和农业农村部:国家现代农业产业技术体系资助”(CARS-21)。

摘　　要：为进一步探究高盐摄入对普通小鼠肾脏的影响,本研究以C57BL/6J小鼠为研究对象,分为正常对照组(NC)、4%高盐组以及8%高盐组,分别喂养NaCl含量为0.5%、4%及8%的饲料,对比小鼠的肾功能指标、炎症因子分泌量以及病理损伤和纤维化程度。结果表明,高盐摄入导致C57BL/6J小鼠的肾功能损伤,显著性提高血清肌酐、血清尿素氮和尿液钠含量,显著降低尿液肌酐含量,同时,8%高盐摄入比4%高盐摄入能进一步加重肾脏损伤,包括进一步降低尿液肌酐以及升高血清尿素氮含量。相比于NC组,4%和8%高盐摄入也会提高肾脏炎症因子TNF-α、IL-6和IL-1β的分泌,而且8%高盐组比4%高盐组进一步提高了IL-6和IL-1β的分泌。HE和Masson染色结果显示,高盐摄入使C57BL/6J小鼠肾脏出现纤维化以及皮质和髓质病理损伤,且8%高盐组比4%高盐组进一步加重。高盐摄入会造成C57BL/6J小鼠肾脏损伤以及纤维化病变,提高盐浓度的摄入会进一步加重肾脏损伤与纤维化程度。In order to further explore the effects of high salt intake on kidney of ordinary mice,C57BL/6J mice were randomly allocated into normal control group(NC),4%high salt intake group(4%group)and 8%high salt intake group(8%group),which were fed with 0.5%NaCl normal diet,4%or 8%high salt diet respectively.The renal function index,inflammatory factor secretion,pathological damage and fibrosis degree of mice from different groups were compared.The results indicated that high salt intake led to renal function injury in C57BL/6J mice.The contents of serum creatinine,serum urea nitrogen and urine Na were significantly increased,whereas the content of urine creatinine was significantly decreased.The 8%high salt diet showed further renal damage when compared to the 4%high salt diet,which include further decrease in urine creatinine and an increase in serum urea nitrogen.Compared with NC,4%and 8%high salt intake also increased the secretion of inflammatory cytokines TNF-α、IL-6 and IL-1βin kidney,and the secretion of IL-6 and IL-1βwas further increased in the 8%group compared with the 4%group.As the HE and Masson staining results shown,high salt intake caused renal fibrosis and pathological damage of cortex and medulla in C57BL/6J mice,and the 8%group was more severe than the 4%group.High salt diet induces kidney injury and fibrosis in C57BL/6J mice,and increasing salt intake can further aggravate the severity of injury and fibrosis.

关 键 词：高盐摄入 肾脏损伤 肾脏纤维化 肾脏功能 炎症因子 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]