cSN50.1对SGC-7901细胞中MMP-9和IL-1β影响的研究  

Effects of cSN50.1 on MMP-9 and IL-1βin SGC-7901 cells

作　　者：李宜澤 吉夫 高建伟 张百强 伍绍鹏 卜月 栾海艳[1,2] LI Yi-ze;ANKOUMA LIGUINEBANDI GUY FERNAND;GAO Jian-wei;ZHANG Bai-qiang;WU Shao-peng;BU Yue;LUAN Hai-yan(Physiology Department of Basic Medical School,Jiamusi University,Jiamusi154007,China;Key Laboratory of Microecology-immune Regulatory Networkand Related Diseases,Jiamusi University,Jiamusi154007,China)

机构地区：[1]佳木斯大学基础医学院生理教研室,黑龙江佳木斯154007 [2]佳木斯大学微生态-免疫调节网络与相关疾病重点实验室,黑龙江佳木斯154007

出　　处：《解剖科学进展》2022年第5期573-576,580,共5页Progress of Anatomical Sciences

基　　金：黑龙江省自然科学基金联合引导项目(LH2019H060);黑龙江省卫生计生委项目(20210404010187);国家级大学生创新创业项目(202010222059);黑龙江省北药与功能食品特色学科项目(2018-TSXK-02)。

摘　　要：目的研究cSN50.1对SGC-7901细胞中MMP-9和IL-1β表达的影响。方法采用CCK-8、细胞划痕、Transwell技术检测不同浓度cSN50.1对SGC-7901细胞增殖、侵袭和迁移的影响,采用RT-PCR和Western blot技术检测cSN50.1对SGC-7901细胞中MMP-9和IL-1β基因和蛋白表达的影响。结果一定浓度的cSN50.1能抑制SGC-7901细胞增殖、迁移和侵袭,且抑制程度随cSN50.1浓度增加而加强;cSN50.1还能够降低SGC-7901细胞中MMP-9和IL-1β的基因和蛋白表达水平。结论cSN50.1可能是通过抑制MMP-9和IL-1β表达来影响SGC-7901细胞的增殖、迁移和侵袭。Objective To study the effect of cSN50.1 on the expression of MMP-9 and IL-1β in SGC-7901gastric cancer cells.Methods The effects of different concentrations of cSN50.1 on the proliferation,invasion and migration of SGC-7901 cells were detected by CCK-8,cell scratch,and Transwell techniques.The expressions of MMP-9and IL-1β were detected by RT-PCR and Western blot techniques.The effects of cSN50.1 on gene and protein expression of MMP-9 and IL-1β in SGC-7901 cells were detected by RT-PCR and Western blot.Results A certain concentration of cSN50.1 can inhibit the proliferation,migration and invasion of SGC-7901 cells,and the degree of inhibition increases with the concentration of cSN50.1.cSN50.1 was also able to reduce the gene and protein expression levels of MMP-9 and IL-1βin SGC-7901 cells.Conclusion cSN50.1 may affect the proliferation,migration and invasion of SGC-7901 cells by inhibiting the expression of MMP-9 and IL-1β.

关 键 词：胃癌 cSN50.1 基质金属蛋白酶-9 白细胞介素-1Β 

分 类 号：R735.2[医药卫生—肿瘤]