miR-194-5p通过FOXP2影响骨肉瘤SAOS-2细胞增殖和侵袭的研究  

Effect of over-expression of miR-194-5p on proliferation and invasion of human osteosarcoma SAOS-2 cells

作　　者：李鹏程 王楠[1] 董宇 张洋[1] LI Peng-cheng;WANG Nan;DONG Yu;ZHANG Yang(The 4th Department of Orthopaedic,the Central Hospital Affiliated to Shenyang Medical College,Shenyang 110024,China)

机构地区：[1]沈阳医学院附属中心医院骨外四科,辽宁沈阳110024

出　　处：《解剖科学进展》2022年第5期585-588,共4页Progress of Anatomical Sciences

基　　金：辽宁省科学技术计划项目(2019-ZD-0321)。

摘　　要：目的探讨高表达miR-194-5p对骨肉瘤SAOS-2细胞增殖和侵袭的影响及机制。方法实时荧光定量PCR方法检测骨肉瘤SAOS-2细胞与人成骨hFOB1.19细胞中miR-194-5p的表达水平,应用瞬时转染将miR-194-5p模拟物转染至骨肉瘤SAOS-2细胞,实时荧光定量PCR检测各组细胞中miR-194-5p、FOXP2 mRNA的相对表达水平,Western blot检测各组细胞中FOXP2蛋白表达,Targetscan7.2和双荧光素酶实验分析FOXP2与miR-194-5p的靶向关系,采用CCK8法检测各组细胞的增殖能力,transwell实验检测细胞侵袭能力。结果miR-194-5p在骨肉瘤SAOS-2细胞中的表达低于人成骨hFOB1.19细胞(P<0.05);过表达miR-194-5p使SAOS-2细胞中FOXP2mRNA和蛋白表达下降;Targetscan7.2网站分析及双荧光素酶实验证实FOXP2为miR-194-5p的靶基因;过表达miR-194-5p使SAOS-2细胞增殖、侵袭能力下降。结论过表达miR-194-5p可以抑制骨肉瘤SAOS-2细胞增殖能力和侵袭能力,其机制可能与其负调控FOXP2的表达有关。Objective To investigate the effect and mechanism of over-expression of miR-194-5p on the proliferation and invasion of SAOS-2 cells.Methods The expression of miR-194-5p in osteosarcoma SAOS-2 cells and human osteoblasts hFOB1.19 cells was detected by qRT-PCR.miR-194-5p mimics was transfected into SAOS-2 cells.The level of miR-194-5p and FOXP2 mRNAin each group were detected by qRT-PCR.The expression of FOXP2 was detected by Western blot.The targeting relationship between FOXP2 and miR-194-5p was analyzed by targetscan7.2 and double luciferase experiment.The proliferation ability of cells in each group was detected by CCK8 method.The invasion ability of cells was detected by transwell assay.Results The level of miR-194-5p in osteosarcoma SAOS-2 cells was lower than that in human osteoblast hFOB1.19 cells(P<0.05).Over-expression of miR-194-5p decreased the expression of FOXP2 in SAOS-2 cells.Targetscan7.2 analysis and double luciferase experiment confirmed that FOXP2 was the target gene of miR-194-5p.Over-expression of miR-194-5p could inhibit the proliferation and invasion of SAOS-2 cells.Conclusion Over-expression of miR-194-5p could inhibit the proliferation and invasion of SAOS-2 cells,which may be related to the regulation of FOXP2 expression.

关 键 词：miR-194-5p SAOS-2细胞 增殖 侵袭 

分 类 号：R738.1[医药卫生—肿瘤]