Temporal profiling with ultra-deep RRBS sequencing reveals the relative rarity of stably maintained methylated CpG sites in human cells  

作　　者：Jianing Wang Yulan Qin Yani Kang Xinhui Li Yuan Wang Hua Li Daniel M.Czajkowsky Zhifeng Shao 

机构地区：[1]State Key Laboratory for Oncogenes and Bio-ID Center,School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Acta Biochimica et Biophysica Sinica》2022年第12期1935-1938,共4页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Key R&D Program of China(No.2020YFA0908100);the National Natural Science Foundation of China(Nos.31670722,81627801,81972909,and 31971151);the Natural Science Foundation of Shanghai(No.19ZR1476100);the Interdisciplinary Program of Medical Engineering Cross Fund(Nos.YG2019GD02,YG2019QNA52 and ZH2018ZDB02);the K.C.Wong Education Foundation(H.K.).

摘　　要：Methylation of cytosine within cytosine-guanine(CpG)sites is one of the most common forms of epigenetic regulation of genomic processes in mammals[1].As such,faithful transmission of CpG methylation following DNA replication is believed to be essential for the maintenance of cell phenotype and function[2].However,it is also known that DNA methylation is a dynamic process at a number of CpG sites during cell passaging.In fact,partial methylation is quite common at the population level,implying that there is considerable heterogeneity in methylation at many CpG sites among individual cells in the population.Thus,it is possible that only a portion of all CpG sites must remain methylated in all of the cells in the population to maintain the phenotypic identity and functional state of each cell.

关 键 词：CPG maintained MAINTAIN 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]