野番豆茎的化学成分研究  

Study on the chemical constituents of the stalk of Uraria clarkei Gagnep

作　　者：张丽[1] 肖健 王煜丹 席晓萌 田凯[1,2] 孙静贤[1,2] 黄相中[1,2] 王韦[1,2] ZHANG Li;XIAO Jian;WANG Yu-dan;XI Xiao-meng;TIAN Kai;SUN Jing-xian;HUANG Xiang-zhong;WANG Wei(Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission and Ministry of Education of China,Yunnan Minzu University,Kunming 650500,China;Laboratory of Theoretical and Computational Chemistry,Yunnan Minzu University,Kunming 650500,China)

机构地区：[1]云南民族大学民族药资源化学国家民族事务委员会-教育部重点实验室,云南昆明650500 [2]云南民族大学理论与计算化学实验室,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2023年第1期1-5,共5页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(81960783);云南省高校传统彝药药效物质基础研究科技创新团队项目.

摘　　要：利用色谱法和重结晶的方法从野番豆茎的95%乙醇提取物的石油醚部分和正丁醇部分中分离得到8个化合物,结合化合物的理化性质及波谱数据,将化合物的结构依次确定为:β-谷甾醇(1),豆甾醇(2),亚油酸甘油单酯(3),牡荆素(4),4′-羟基-7-O-β-D-葡萄糖-2,3-二氢黄酮苷(5),柚皮苷(6),橙皮素(7),高北美圣草素(8),其中化合物4~8为黄酮类化合物,化合物1~8均为首次从该种植物中分离得到.对化合物4~8进行了α-葡萄糖苷酶抑制活性筛选,结果表明,化合物4、7及8对α-葡萄糖苷酶具有抑制作用,IC 50值分别为(94.92±4.37)、(41.40±3.68)及(38.70±3.32)μmol/L.Eight compounds were isolated from the petroleum ether fraction and the n-butanol fraction of 95%ethanol extracts of Uraria clarkei Gagnep by chromatography and recrystallization.Compounds structures were elucidated by spectroscopic methods and physicochemical properties.The eight compounds obtained were identified asβ-sitosterol(1),stigmasterol(2),monolinoleoyl glycerol(3),vitexin(4),4′-hydroxy-2,3-dihydroflavonone 7-O-β-D-glucoside(5),naringin(6),hesperetin(7),homoeriodictyol(8),and compounds 4~8 are flavonoids.The compounds 1~8 were obtained from this plant for the first time.In anα-glucosidase inhibitory assay,compounds 4,7 and 8 exhibitedα-glucosidase inhibitory activity with IC 50 values of(94.92±4.37),(41.40±3.68),(38.70±3.32)μmol/L,respectively.

关 键 词：野番豆 黄酮类化合物 甾体 Α-葡萄糖苷酶 

分 类 号：R284.1[医药卫生—中药学]