Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity  

作　　者：Ming Xia Md Rejaul Hoq Pengwei Huang Wen Jiang Xi Jiang Ming Tan 

机构地区：[1]Division of Infectious Diseases,Cincinnati Children’s Hospital Medical Center,Cincinnati,OH 45229,USA [2]Department of Biological Sciences,Purdue Cryo-EM Facility,Purdue University,IN 47907,USA [3]Department of Pediatrics,University of Cincinnati College of Medicine,Cincinnati,OH 45229,USA

出　　处：《Nano Research》2022年第5期4181-4190,共10页纳米研究（英文版）

基　　金：The research described in this study was supported by the National Institute of Allergy and Infectious Diseases(NIAID,No.R56 AI148426-01A1 to M.T.);Cincinnati Children Hospital Medical Center(CCHMC,Innovation Funds 2018-2020,GAP Fund 2020-2021,and Research Innovation and Pilot Grant 2020-2021 to M.T.);the Center for Clinical and Translational Science and Training(CCTST)of the University of Cincinnati College of Medicine(Pilot Collaborative Studies Grant 2018-2019 to M.T.);that was supported by the National Center for Advancing Translational Sciences of the National Institutes of Health(No.UL1TR001425).

摘　　要：Even with implementation of current influenza vaccines,influenza still claims up to 500,000 lives worldwide annually,indicating a need for a better vaccine strategy.We have developed a technology to generate unique S_(60)-HA1 pseudovirus nanoparticles(PVNPs)that display the receptor-binding HA1 domains of influenza viruses.Each self-assembled S_(60)-HA1 PVNP consists of a T=1 icosahedral S_(60) nanoparticle that resembles the inner shell of norovirus capsid and 60 surface-displayed HA1 antigens that are excellent vaccine targets.Soluble S_(60)-HA1 PVNPs presenting HA1 antigens of H7N9 influenza virus subtypes have been produced efficiently in large amount.Their three-dimensional(3D)structures have been solved by cryogenic electron microscopy.The PVNP-displayed HA1 antigens react with HA-specific antibody,and retain authentic sialic acid binding specificity and hemagglutinate human erythrocytes.The PVNPs are highly immunogenic,eliciting high titers of HA1-specific antibodies in mice and the mouse sera strongly inhibited hemagglutinations of homologous and heterologous influenza virus HA proteins.Therefore,the S_(60)-HA1 PVNPs may provide useful reagents to study influenza viruses and offer a potential new vaccine tactic to fight the deadly influenza disease.

关 键 词：S_(60)nanoparticle pseudovirus nanoparticle influenza virus influenza vaccine HEMAGGLUTININ NOROVIRUS 

分 类 号：R37[医药卫生—病原生物学] TB383[医药卫生—基础医学]