HPLC法同时测定地顶孢霉培养物中腺苷、虫草素和麦角甾醇含量  

Simultaneous determination of adenosine,cordycepin and ergosterol in Acremoniumterricola culture by HPLC

作　　者：刘书余 陈晋元 孙清平 郭亚文[1,2] 管凡荀 高鹏飞 汤亚云 朱雅丽 谢恺舟 LIU Shuyu;CHEN Jinyuan;SUN Qingping;GUO Yawen;GUAN Fanxun;GAO Pengfei;TANG Yayun;ZHU Yali;XIE Kaizhou(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;Joint International Research Laboratory of Agriculture and Agri-Product Safety,Yangzhou University,Yangzhou 225009,China;METDO(Jiangsu)Biotechnology Company Limited,Yangzhou 225106,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]扬州大学教育部农业与农产品安全国际合作联合实验室,江苏扬州225009 [3]迈特多(江苏)生物科技有限公司,江苏扬州225106

出　　处：《扬州大学学报（农业与生命科学版）》2022年第6期111-117,132,共8页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家现代农业产业技术体系专项(CARS-41);江苏省高校优势学科建设工程项目(PAPD)。

摘　　要：为建立同时测定地顶孢霉培养物中腺苷、虫草素和麦角甾醇含量的高效液相色谱法,选择色谱柱为Waters C18柱(4.6 mm×250 mm,5μm),流动相为水-乙腈,梯度洗脱,检测波长270 nm,流速1 mL·min^(-1),柱温30℃,进样量20μL。结果表明:腺苷、虫草素和麦角甾醇在浓度20~640μg·mL-1(X)范围内与峰面积(Y)呈良好的线性关系,腺苷、虫草素和麦角甾醇的回归方程分别为Y=98.53X+586.26(R^(2)=0.9993)、Y=124.16X+277.69(R^(2)=0.9997)、Y=57.652X+107.47(R^(2)=0.9996)。腺苷、虫草素和麦角甾醇的加标回收率分别为95.96%~99.95%、97.43%~99.94%和96.31%~99.95%。该方法准确度高、重复性好、灵敏度高,适用于地顶孢霉培养物中腺苷、虫草素和麦角甾醇含量的测定,为其质量监控提供了技术支撑。A high performance liquid chromatography(HPLC)method was established for the simultaneous determination of adenosine,cordycepin and ergosterol in Acremoniumterricola culture.Separation was completed in Waters C18 column(4.6 mm×250 mm,5μm).Mobile phase was water-acetonitrile.Detection wavelength was set at 270 nm.The flow rate was 1 mL·min^(-1).The column temperature was controlled at 30℃,and the injection volume was 20μL.The calibration curve was good linear between the ratio of the peak areas(Y)and concentrations(X)of adenosine,cordycepin and ergosterol from 20 to 640 ng·mL-1.The regression equations of adenosine,cordycepin and ergosterol were Y=98.53X+586.26,Y=124.16X+277.69,Y=57.652X+107.47,respectively.The R^(2)values were higher than 0.9993.The average recovery rate of adenosine,cordycepin and ergosterol was 95.96%-99.95%,97.43%-99.94%and 96.31%-99.95%,respectively.This method had characteristics of high accuracy,good repeatability and sensitivity,which was suitable for the determination of adenosine,cordycepin and ergosterolin Acremoniumterricolaculture and provide technical support for its quality control.

关 键 词：地顶孢霉培养物 腺苷 虫草素 麦角甾醇 高效液相色谱法 同时测定 

分 类 号：O657.7[理学—分析化学]