三阴性乳腺癌组织长链非编码RNA-P21、microRNA-17-3p的表达及其临床意义  

Expression and clinical significance of LncRNA-P21 and microRNA-17-3p in triple negative breast cancer tissues

作　　者：刘凡[1] 施文瑜[1] 刘益飞[2] 王国华[3] Liu Fan;Shi Wen-yu;Liu Yi-fei;Wang Guo-hua(Department of Oncology and Chemotherapy,Affiliated Hospital of Nantong University,Nantong,Jiangsu 226001,China;Department of Tumor Pathology,Affiliated Hospital of Nantong University,Nantong,Jiangsu 226001,China;Special Research Institute of Nantong University,Nantong,Jiangsu 226001,China)

机构地区：[1]南通大学附属医院肿瘤化疗科,江苏南通226001 [2]南通大学附属医院病理科,江苏南通226001 [3]南通大学特种研究所,江苏南通226001

出　　处：《中国现代医学杂志》2023年第5期16-22,共7页China Journal of Modern Medicine

基　　金：江苏省重点研发计划(No:BE2018778);江苏省第五期“333工程”科研项目(No:BAR2019207)。

摘　　要：目的探讨三阴性乳腺癌组织中长链非编码RNA-P21(LncRNA-P21)、microRNA-17-3p(miR-17-3p)的表达,分析其与三阴性乳腺癌患者的临床病理特征及预后的关系。方法选取2016年1月—2017年5月南通大学附属医院收治的106例三阴性乳腺癌患者经手术切除的癌组织和癌旁组织(距离癌组织至少5 cm)标本,采用实时荧光定量聚合酶链反应检测癌组织、癌旁组织中LncRNA-P21和miR-17-3p的表达;收集患者的临床病理特征资料,分析LncRNA-P21、miR-17-3p表达与临床病理特征的关系;术后随访5年,采用Kaplan-Meier法绘制不同LncRNA-P21、miR-17-3p表达三阴性乳腺癌患者的生存曲线;Cox回归分析影响三阴性乳腺癌患者预后的因素。结果癌组织LncRNA-P21 mRNA相对表达量低于癌旁组织(P<0.05);癌组织miR-17-3p mRNA相对表达量高于癌旁组织(P<0.05)。临床Ⅲ期、腋窝淋巴结转移、Ki-67≥30%三阴性乳腺癌组织中LncRNA-P21 mRNA相对表达量低于临床Ⅰ、Ⅱ期,无腋窝淋巴结转移和Ki-67<30%(P<0.05);临床Ⅲ期、腋窝淋巴结转移、Ki-67≥30%三阴乳腺癌组织中miR-17-3p mRNA相对表达量高于临床Ⅰ、Ⅱ期,无腋窝淋巴结转移和Ki-67阴性表达(P<0.05)。不同年龄、肿瘤直径、分化程度、绝经状态的三阴性乳腺癌患者LncRNA-P21、miR-17-3p mRNA相对表达量比较,差异无统计学意义(P>0.05)。三阴性乳腺癌组织中LncRNA-P21表达与miR-17-3p表达呈负相关(r=-0.570,P<0.05)。LncRNA-P21低表达组5年无病生存期(DFS)和总生存期(OS)生存率低于LncRNA-P21高表达组(P<0.05),miR-17-3p高表达组5年DFS、OS生存率低于miR-17-3p低表达组(P<0.05)。单因素Cox回归分析结果显示,分化程度、TNM分期、腋窝淋巴结转移、LncRNA-P21、miR-17-3p是三阴性乳腺癌患者预后的影响因素(P<0.05);多因素Cox风险比例回归分析结果显示,腋窝淋巴结转移、miR-17-3p是三阴性乳腺癌患者预后不良的危险因素(P<0.05),LncRNA-P21是保护因素(PObjective To investigate the relationship of LncRNA-P21,microRNA-17-3p(miR-17-3p)expression with clinicopathological features and prognosis in triple-negative breast cancer.Methods From January 2016 to May 2017,106 patients with triple-negative breast cancer who underwent surgical resection of cancer tissues and adjacent tissues(at least 5 cm away from cancer tissues)were selected.Real-time quantitative polymerase chain reaction was used to detect the expression of LncRNA-P21 and miR-17-3p in tissue samples.Clinicopathological data of patients were collected,and the relationship between LncRNA-P21,miR-17-3p expression and clinicopathological features of patients was analyzed.After 5 years of follow-up,Kaplan-Meier method was used to draw the survival curves of triple-negative breast cancer under different expressions of LncRNA-P21 and miR-17-3p.Cox regression analysis was used to analyze the factors affecting the prognosis of triple negative breast cancer patients.Results The expression of LncRNA-P21 in cancer tissue was lower than that in adjacent tissues(P<0.05);The expression of miR-17-3p in cancer tissue was higher than that in adjacent tissues(P<0.05).The expression of LncRNA-P21 in triple-negative breast cancer tissue with TNM Ⅲ,axillary lymph node metastasis,and Ki-67≥30% was lower than that in TNM Ⅰ-Ⅱ,no axillary lymph node metastasis,and Ki-67<30%(P<0.05),and the expression of miR-17-3p was higher than that in TNM Ⅰ-Ⅱ,no axillary lymph node metastasis,and Ki-67negative(P<0.05).There was no significant difference in the expression of LncRNA-P21 and miR-17-3p in different age,tumor diameter,degree of differentiation,and menopausal status(P>0.05).The expression of LncRNA-P21 was negatively correlated with the expression of miR-17-3p in triple negative breast cancer(r=-0.570,P<0.05).The disease-free survival(DFS)and total survival(OS)survival rates in the low expression group of LncRNA-P21 were lower than those in the high expression group of LncRNA-P21(P<0.05).The survival rate of DFS and OS in

关 键 词：三阴性乳腺癌 长链非编码RNA-P21 microRNA-17-3p 病理 预后 

分 类 号：R737.9[医药卫生—肿瘤]