hsa_circ_0000073对骨肉瘤脂质代谢的影响  

Effect of hsa_circ_0000073 on lipid metabolism in osteosarcoma

作　　者：任智晶 杨震[2] 杨丹 田晓滨 REN Zhijing;YANG Zhen;YANG Dan;TIAN Xiaobin(Department of Clinical Laboratory,Guizhou Provincial People’s Hospital,Guiyang,Guizhou 550002,China;Department of Orthopedics,Guizhou Provincial People’s Hospital,Guiyang,Guizhou 550002,China;Department of Orthopedics,Affiliated Hospital of Guizhou Medical University,Guiyang,Guizhou 550000,China)

机构地区：[1]贵州省人民医院临床检验科,贵阳550002 [2]贵州省人民医院骨科,贵阳550002 [3]贵州医科大学附属医院骨科,贵阳550000

出　　处：《重庆医学》2023年第5期641-647,共7页Chongqing medicine

基　　金：国家自然科学基金项目(31960208、81960536);贵州省人民医院青年基金项目[GZSYQN(2019)15]。

摘　　要：目的探讨hsa_circ_0000073在骨肉瘤组织中的表达及其对骨肉瘤细胞脂质代谢的影响。方法收集2020年1月至2022年1月贵州省人民医院就诊且经病理证实的10例骨肉瘤患者的肿瘤组织及癌旁组织,通过实时荧光定量PCR(qRT-PCR)检测hsa_circ_0000073的表达水平。骨肉瘤细胞转染sh-NC和sh-hsa_circ_0000073,通过G418构建稳转细胞株,荧光显微镜和qRT-PCR验证干扰效率。使用GeneChip基因芯片筛选出差异表达基因并进行GO分析,R语言(3.6.3版本)的“clusterProfiler”包用于数据分析,“ggplot2”包用于数据可视化。骨肉瘤细胞和裸鼠异种移植双重模型上进行油红实验、尼罗红实验评估hsa_circ_0000073对骨肉瘤细胞脂质代谢的影响。结果与癌旁组织比较,骨肉瘤组织hsa_circ_0000073表达水平升高(P<0.05)。转染sh-RNAs后,circ_0000073干扰组hsa_circ_0000073表达水平低于对照组(P<0.05)。干扰hsa_circ_0000073后,GeneChip表达谱芯片筛选出的差异基因主要富集于有丝分裂核分裂、纺锤体、染色体、着丝粒区,而“代谢通路”是富集到的主要通路。circ_0000073干扰组的骨肉瘤细胞和裸鼠异种移植瘤体中的红色脂质和尼罗红荧光强度均低于对照组(P<0.05)。结论hsa_circ_0000073在骨肉瘤中高表达并与脂质代谢有关。Objective To investigate the expression of hsa_circ_0000073 in osteosarcoma tissues and its effect on lipid metabolism in osteosarcoma cells.Methods Tumor tissues and paracancer tissues of 10 patients with osteosarcoma confirmed by pathology were collected from Guizhou Provincial People’s Hospital from January 2020 to January 2022.The expression level of hsa_circ_0000073 was detected by quantitative real-time PCR(qRT-PCR).Osteosarcoma cells were transfected with sh-NC and sh-hsa_circ_0000073.Stable cell lines were constructed by G418,and the interference efficiency was verified by fluorescence microscopy and qRT-PCR.The GeneChip microarray was used to screen out differentially expressed genes and perform GO and KEGG enrichment analysis was carried out.The“clusterProfiler”package in R language(version 3.6.3)was used for data analysis,and the“ggplot2”package was used for data visualization.The effects of hsa_circ_0000073 on lipid metabolism of osteosarcoma cells were evaluated by oil red assay and Nile red assay in dual model of osteosarcoma cell and nude mice xenotransplantation.Results Compared with paracancer tissue,the expression level of hsa_circ_0000073 in osteosarcoma tumor tissue was increased(P<0.05).After transfection with sh-RNAs,the expression level of hsa_circ_0000073 in circ_0000073 interference group was lower than that in the control group(P<0.05).After interference with hsa_circ_0000073,the differential genes screenedby GeneChip expression profile microarray were mainly enriched in mitotic mitosis,spindle,chromosome and centromeric region,and the“metabolic pathway”was the main pathway enriched.The fluorescence intensity of red lipids and Nile red in osteosarcoma cells and xenografts of nude mice in circ_0000073 interference group was lower than that in the control group(P<0.05).Conclusion hsa_circ_0000073 is highly expressed in osteosarcoma and is associated with lipid metabolism.

关 键 词：骨肉瘤 hsa_circ_0000073 基因芯片 代谢通路 脂质代谢 

分 类 号：R738.6[医药卫生—肿瘤]