基于RNA-seq的绿芦笋嫩茎冷藏期间茉莉酸信号变化研究  

Changes of JA Signaling in Spears of Green Asparagus during Cold Storage Based on RNA-seq

作　　者：付永琦 费柳莹 钟丽 李衍苏 陈金印 FU Yongqi;FEI Liuying;ZHONG Li;LI Yansu;CHEN Jinyin(College of Agronomy,Jiangxi Key Laboratory for Postharvest Technology and Non-destructive Testing of Fruits&Vegetables,Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province,Jiangxi Agricultural University,Nanchang 330045,China;College of Horticulture,South China Agricultural University,Guangzhou 510000,China)

机构地区：[1]江西农业大学农学院/江西省果蔬保鲜与无损检测重点实验室/江西省果蔬采后处理关键技术与质量安全协同创新中心,江西南昌330045 [2]华南农业大学园艺学院,广东广州510000

出　　处：《江西农业大学学报》2023年第1期101-112,共12页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31860581);江西省科技支撑计划项目(20133BBF60009)。

摘　　要：【目的】为探讨茉莉酸(JA)对绿芦笋采后品质变化进程的影响及其分子机制,对绿芦笋采后冷藏过程中JA含量与品质变化之间的关系以及不同冷藏时期转录组数据进行挖掘分析。【方法】测定绿芦笋冷藏期间品质变化相关指标以及JA含量的变化,采用RNA-seq技术分析绿芦笋5个冷藏时期嫩茎顶部组织的转录组变化,筛选富集于JA生物合成及信号转导途径的差异表达基因(DEGs),并通过实时荧光定量PCR技术对其中4个基因的表达模式进行验证。【结果】随着贮藏时间延长,绿芦笋的感官评分分值显著下降,失重率显著上升,顶部组织的硬度不断下降,基部组织的硬度呈先升后降;贮藏当天呼吸速率极高,但随着贮藏期的延长呈现先降后升的变化模式。JA含量在贮藏前几个小时含量急剧增加,贮藏期间则呈现先降后升的变化模式,且顶部组织JA含量极显著高于基部;5个时期嫩茎顶部组织中富集于茉莉酸生物合成及信号转导途径的差异基因分别有32个和11个,而其中最关键的基因分别有16个和9个。茉莉酸生物合成途径中,上调表达的差异基因主要涉及JA合成前体物质的产生以及下游调控步骤。JA信号转导途径转录调控非常活跃,以JA活化以及识别蛋白COI1的诱导表达为主要特征,JAZ蛋白响应基因数目最多,且其表达模式与JA含量的变化趋势较为一致。【结论】茉莉酸信号变化与绿芦笋采后品质变化密切相关,且有25个关键基因可能参与了这一响应过程,为进一步阐明绿芦笋采后品质变化的分子机制奠定理论基础。[Objective]In order to explore the effects and molecular mechanism of Jasmonic acid(JA)on the process of postharvest quality change in green asparagus,the relationship between the content of JA and the quality of green asparagus during the process of cold storage was analyzed,and the transcriptome data of different cold storage periods were mined.[Method]In this study,based on the analysis of some indicators related to quality changes and JA content changes of green asparagus during cold storage. Transcriptome changes in the top of spears from five cold storage periods were analyzed by RNA-seq technique.And then the differentially expressed genes(DEGs)enriched in JA biosynthesis and JA signal transduction pathways were screened.Finally,four of these DEGs were verified by real-time fluorescence quantitative PCR.[Result]This study showed that the sensory score decreased significantly and the weight loss rate increased significantly with the extension of storage time,and the firmness of the top spear decreases continuously,while the firmness of the base spear increased firstly and then decreases.Before cold storage,the respiratory rate was very high,but it decreased firstly,then increased during cold storage.JA content increased sharply in a few hours before storage,and presented a change pattern of decreasing firstly and then increasing during storage.JA content of the top tissue was significantly higher than that of the base tissue.Comparing five transcriptomes data of storage time with transcriptomes data of the day of start storage,32 DEGs referred to JA biosynthesis pathway were identified and 11 DEGs referred to JA signal transducer pathway were identified.Among of them,the most critical genes had 16 DEGs and 9 DEGs,respectively. In JA biosynthetic pathway,DEGs were mainly involved in the production of JA synthetic precursor and downstream regulatory of biosynthesis step.Transcriptional regulation was very active in JA signal transducer pathways,and those DEGs which were induced expression were mainly involved

关 键 词：绿芦笋 贮藏品质 茉莉酸信号 转录组测序 

分 类 号：S644.6[农业科学—蔬菜学]