Evaluation of Uncertainty for Determination of Stevioside Content in Fermented Milk by HPLC  

作　　者：Zhiyong LU Hong YUE Yuelian NING Xiaoyan HUANG Wenhui HU Shuo TANG Yuju REN Lijun LIU Cuizhi LI 

机构地区：[1]Inner Mongolia Yili Industrial Group Co.,Ltd.,Hohhot 010110,China

出　　处：《Asian Agricultural Research》2023年第1期41-46,共6页亚洲农业研究（英文）

摘　　要：[Objectives]The paper was to establish an evaluation method for the uncertainty of stevioside(including stevioside,rebaudioside A,rebaudioside B,rebaudioside C,rebaudioside F,Dulcoside A,rubusoside and steviolbioside)content determination in fermented milk based on HPLC.[Methods]The mathematical model of stevioside content and the propagation rate of uncertainty were established,and the sources of uncertainty were analyzed.[Results]The uncertainty mainly came from four main aspects,including standard uncertainty u(C)introduced by solution concentration C,standard uncertainty u(V)introduced by sample volume V,standard uncertainty u(m)introduced by sample mass m weighing and standard uncertainty u(f_(rep))introduced by measurement repeatability of stevioside content after sample dissolution and constant volume.The uncertainty estimation table and fishbone chart of stevioside content X determination were established.The relative synthetic standard uncertainty of stevioside content was obtained,and the standard uncertainty was extended to form the measurement result of stevioside content and its uncertainty report.[Conclusions]The evaluation results can be directly applied to the daily practical detection work.

关 键 词：HPLC STEVIOSIDE Uncertainty Relative synthetic standard uncertainty Extended standard uncertainty 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]