高温和高盐对Nitrosomonas eutropha CZ-4的交叉保护效应  

Cross-tolerance of Nitrosomonas eutropha CZ-4 to high-temperature and high salinity

作　　者：杨睿 葛红梅[1] 程凯[1] YANG Rui;GE Hong-mei;CHENG Kai(Hubei Key Laboratory of Ecological Restoration for River-Lakes and Algal Utilization,College of Environment and Hydraulic Engineering,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学环境与水利工程学院,河湖生态修复及藻类利用湖北省重点实验室,湖北武汉430068

出　　处：《中国环境科学》2023年第3期1378-1385,共8页China Environmental Science

基　　金：国家科技重大专项(2017ZX07602002)。

摘　　要：为探究高温和高盐对亚硝化单胞菌亚硝化能力的联合影响,以Nitrosomonas eutropha CZ-4为试验材料,测试了不同程度的高温和高盐对其亚硝化能力的短期交叉影响,比较了不同的长期驯化方式对该菌胞外聚合物含量的影响及对该菌响应高盐或高温胁迫的影响.结果表明,当温度从28℃逐步升高至40℃时,亚硝化的最适盐浓度从2g/L逐步升高至18g/L,而且高盐/高温分别能够明显缓解高温/高盐对亚硝化的抑制作用.高盐驯化显著增强了耐热性,高温驯化则显著增强了耐盐性,且高盐驯化和高温驯化后结合型多糖的含量均显著高于低温低盐处理组.上述结果证明高温和高盐对CZ-4具有生理性的交叉保护效应,且高温或高盐均能诱导CZ-4胞外结合型多糖的积累.To study the combined influence of high-temperature and high salinity on the nitrosation activity of Nitrosomonas,the cross-tolerance of Nitrosomonas eutropha CZ-4 to high-temperature and high salinity by short-term cross-experiment at different salinities and temperatures was investigated.Also,stress experiments were performed after long-term preacclimation,and the extracellular polymeric substance content was measured.Results showed the optimal salt concentration increased gradually from 2g/L to 18g/L when the temperature increased from 28℃to 40℃,and high salinity/high-temperature significantly alleviated the inhibition by high-temperature/high salinity,respectively.The heat tolerance was significantly improved by the high salinity preacclimation,and the salt tolerance also significantly improved by the high-temperature preacclimation.After preacclimation in high-temperature or high salinity conditions,the bound-polysaccharide content significantly increased compared that of cells cultured in low-temperature and low-salinity conditions.The results not only prove the physiological cross-tolerance of CZ-4 to high-temperature and high salinity,but also prove the accumulation of the extracellular bound-polysaccharide of CZ-4 at high-temperature or high salinity conditions.

关 键 词：脱氨 AOB 盐度 温度 逆境胁迫 

分 类 号：X172[环境科学与工程—环境科学]