Comparison of biological behavior of lacrimal gland adenoid cystic carcinoma with high-grade transformation cells  

作　　者：Chuan-Li Zhang Li-Min Zhu Xun Liu Mei-Xia Jiang Ting-Ting Lin Yan-Jin He 

机构地区：[1]Oculoplastic and Orbital Disease Department,Tianjin Medical University Eye Hospital,Eye Institute and School of Optometry,Tianjin Key Laboratory of Retinal Functions and Diseases,Tianjin Branch of National Clinical Research Center for Ocular Disease,Tianjin 300384,China [2]Tianjin Eye Hospital Optometry Center,Tianjin 300384,China

出　　处：《International Journal of Ophthalmology(English edition)》2023年第2期163-171,共9页国际眼科杂志（英文版）

基　　金：Supported by the Tianjin Key Medical Discipline(Specialty)Construction Project(No.TJYXZDXK-037A);Tianjin Medical University Eye Hospital。

摘　　要：AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformation(non-HGT)primary cells cultured by non-highgrade transformation tissue in proliferation,metastasis,drug susceptibility,and genes.METHODS:LACC-HGT primary cells were established by tissue block culture,and the 4^(th)to 10^(th)generation primary cells were selected as research objects.The cells were preliminarily identified by immunofluorescent staining.The differences between non-HGT and LACC-HGT primary cells in terms of proliferation,metastasis,and drug susceptibility were compared by cell counting kit-8(CCK-8)assay,wound healing,and drug sensitivity experiments.Differentially expressed genes were screened using mRNA array.Gene expression was analyzed using real-time quantitative polymerase chain reaction(RT-qPCR).RESULTS:LACC-HGT primary cells were successfully cultured by tissue block culture.Immunofluorescence staining results showed that cytokeratin(CK)and CK7 expression levels were positive in LACC-HGT primary cells.CCK-8 results showed that the proliferation ability of LACCHGT cells was significantly higher than that of non-HGT cells.Wound healing experiment showed that the migration ability of LACC-HGT cells was significantly higher than that of non-HGT cells.LACC-HGT cells were also less sensitive to cisplatin and paclitaxel than non-HGT cells.Compared with non-HGT cells,9566 differentially expressed genes were found in LACC-HGT primary cells,of which 5162 were upregulated and 4404 were down-regulated.The expression of N-acetylneuraminate pyruvate lyase(NPL),MARVEL domain containing 3(MARVELD3),syntabulin(SYBU),and allograft inflammatory factor 1(AIF1)was higher in LACCHGT cells than in non-HGT cells,whereas that of periostin(POSTN)was lower.CONCLUSION:LACC-HGT primary cells have faster proliferation,stronger migration ability,and poorer sensitivity to chemotherapy drugs than non-HGT prima

关 键 词：lacrimal gland lacrimal gland adenoid cystic carcinoma high-grade transformation primary cell culture biological behavior mRNA array 

分 类 号：R739.7[医药卫生—肿瘤]