电针督脉经穴对血管性痴呆大鼠海马CA1区ATF6/CHOP通路的影响  

Effect of electro-acupuncture at Du meridian on ATF6/CHOP pathway in the hippocampal CA1 region of rats with vascular dementia

作　　者：刘芳[1] 李梦醒[2] 王玉 Liu Fang;Li Mengxing;Wang Yu(Anqing Medical College,Anqing 246052,Anhui,China;School of Acupuncture-moxibustion and Tuina,Anhui University of Chinese Medicine,Hefei 230012,Anhui,China;School of Physical Education,Anqing Normal University,Anqing 246113,Anhui,China)

机构地区：[1]安庆医药高等专科学校,安徽安庆246052 [2]安徽中医药大学针灸推拿学院,安徽合肥230012 [3]安庆师范大学体育学院,安徽安庆246113

出　　处：《右江民族医学院学报》2023年第1期39-45,共7页Journal of Youjiang Medical University for Nationalities

基　　金：安徽省高校自然科学研究项目(KJ2019A1233,KJ2020A0377);安徽省高校优秀青年人才支持项目(gxyqZD2022109)。

摘　　要：目的观察电针督脉经穴对血管性痴呆大鼠(vascular dementia,VD)海马CA1区转录激活因子6(ATF6)/内质网应激增强子结合(C/EBP)同源蛋白(CHOP)通路的影响,探讨电针治疗VD的作用机制。方法将SPF级雄性SD大鼠随机分为正常组(Normal组)、假手术组(Sham组)、模型组(Model组)、电针督脉经穴组(EA+督脉经穴组)和电针非经非穴组(EA+非经非穴组),每组12只。除Normal组、Sham组外,其余组均采用双侧颈总动脉永久结扎法复制动物模型。EA+督脉经穴组和EA+非经非穴组给予疏密波、频率15 Hz电针治疗20分钟/次,隔日1次,连续进行4周。Morris水迷宫实验检测大鼠学习、记忆能力。透射电镜观察海马CA1区神经元超微结构。Western Blot法和RT-PCR法检测海马CA1区GRP78、ATF6、CHOP蛋白及其mRNA的表达。结果与Sham组比较,Model组平均逃避潜伏期延长、穿越平台次数减少(P<0.01);海马CA1区神经元细胞结构损坏明显;GRP78、ATF6、CHOP蛋白及mRNA表达明显增高(P<0.01)。与Model组比较,EA+督脉经穴平均逃避潜伏期缩短、穿越平台次数增加(P<0.01);海马CA1区神经元细胞损伤程度改善。GRP78、ATF6、CHOP蛋白及mRNA表达均减少(P<0.01)。与EA+督脉经穴组比较,EA+非经非穴组逃避潜伏期延长,穿越平台次数减少(P<0.01);神经元细胞损伤严重。GRP78、ATF6、CHOP蛋白及其mRNA表达增高(P<0.01)。结论针刺督脉经穴能够改善血管性痴呆大鼠认知障碍其机制可能是通过调控ATF6/CHOP通路,抑制ERS从而发挥其脑保护效应。Objective To observe the effect of electro-acupuncture at Du meridian(EDM)on the ATF6(activating transcription factor 6)/CHOP(C/EBP homologous protein)pathway in the hippocampal CA1 region of rats with vascular dementia(VD),so as to explore the mechanism of electro-acupuncture in the treatment of VD.Methods SPF-grade SD rats were randomly divided into four groups with 12 rats in each group:Normal group,Sham operation group,Model group,EA+Du meridian group and EA+non-meridian and non-acupoint group.Except for the normal group and the sham group,the animal models were duplicated by permanent ligation of bilateral common carotid arteries in the other groups.The EA+Du meridian group and the EA+non-meridian and non-acupoint group were treated with electro-acupuncture of sparse-dense wave with 15 Hz frequency for 20 minutes once every other day for 4 weeks.The learning and memory ability of the rats was tested by the Morris water maze.Transmission electron microscopy was used to observe the ultrastructure of neurons in the hippocampal CA1 region.Western Blot and RT-PCR were adopted to detect the protein and mRNA expressions of GRP78,ATF6 and CHOP in the hippocampal CA1 region.Results Compared with the sham group,the model group had prolonged average escape latency and decreased times of platform crossing(P<0.01).And in the model group,the neuronal structure in the hippocampal CA1 region was significantly damaged;the protein and mRNA expressions of GRP78,ATF6 and CHOP had significantly increased(P<0.01).Compared with the model group,the EA+Du meridian group had shortened average escape latency and increased times of platform crossing(P<0.01).In the the EA+Du meridian group,the degree of neuronal cell damage was alleviated in the hippocampal CA1 region;the protein and mRNA expressions of GRP78,ATF6 and CHOP were decreased(P<0.01).Compared with the EA+Du meridian group,the EA+non-meridian and non-acupoint group had prolonged escape latency and decreased times of platform crossing(P<0.01).And for them,the neuronal cells we

关 键 词：痴呆 血管性 电针 督脉经穴 

分 类 号：R749.16[医药卫生—神经病学与精神病学]