多发性骨髓瘤患者FISH检测结果与Durie-Salmon分期和R-ISS分期的相关性  

Correlation analysis of FISH results with Durie-Salmon staging and R-ISS staging in patients with multiple myeloma

作　　者：廖彩翔[1] 黎国伟[1] 何维清 LIAO Caixiang;LI Guowei;HE Weiqing(Department of Hematology,Huizhou Central People's Hospital,Huizhou,Guangdong,China,516000;Department of Oncology,Huiyang Sanhe Hospital,Huizhou,Guangdong,China,516200)

机构地区：[1]惠州市中心人民医院血液内科,广东惠州516000 [2]惠阳三和医院肿瘤内科,广东惠州516200

出　　处：《分子诊断与治疗杂志》2023年第1期136-140,共5页Journal of Molecular Diagnostics and Therapy

基　　金：惠州市科技计划项目(2021WC0106356)。

摘　　要：目的 探讨多发性骨髓瘤(MM)患者荧光原位杂交(FISH)检测结果与Durie-Salmon分期(DS)和国际分期系统(R-ISS)的关系。方法 选取惠州市中心人民医院2020年1月至2021年1月收治的MM患者80例为研究对象,利用FISH技术检测患者CKS1B(1q21)、p53、RB1、IGH基因表达情况,分析FISH检测结果与DS分期和R-ISS分期的关系。单因素及Cox回归分析MM患者预后的独立危险因素。结果 DS分期和R-ISS分期越高,FISH检测结果阳性发生率越高,不同DS分期、R-ISS分期分别与FISH检测结果比较,差异均有统计学意义(P<0.05)。单因素显示,治疗方案、1q21扩增、p53缺失、RB1缺失、IGH重排与患者预后转归比较,差异均有统计学意义(P<0.05)。Cox回归分析显示,1q21扩增、p53缺失、RB1缺失、IGH重排为MM患者预后结局的独立影响因素(P<0.05),其中,1q21扩增、p53缺失、RB1缺失、IGH重排阳性者1年生存率均低于阴性者。结论 FISH检测结果阳性发生率越高,DS分期和R-ISS分期则越高,且1q21扩增、p53缺失、RB1缺失、IGH重排为MM患者预后结局的独立影响因素。Objective To investigate the relationship between the results of fluorescence in situ hybridization(FISH)and Durie-Salmon staging(DS)and International Staging System(R-ISS)in patients with multiple myeloma(MM). Methods A total of 80 patients with MM who were admitted to Huizhou Central People’s Hospital from January 2020 to January 2021 were selected as the research subjects. The gene expression of CKS1B(1q21),p53,RB1 and IGH was detected by FISH technology,and the correlation between FISH detection results and DS was analyzed. The relationship between staging and R-ISS staging.Univariate and Cox regression analysis of independent risk factors for the prognosis of MM patients. Results The higher the DS stage and the R-ISS stage,the higher the positive incidence rate of FISH test results. The differences among different DS stages,R-ISS stages and FISH test results were statistically significant(P<0.05). Single factor showed that there were statistically significant differences in treatment regimen,1q21amplification,p53 deletion,RB1 deletion,and IGH rearrangement with the prognosis of patients(P<0.05).Cox regression analysis showed that 1q21 amplification,p53 deletion,RB1 deletion,and IGH rearrangement were independent prognostic factors for MM patients(P<0.05). Among them,the 1-year survival rate of patients with positive 1q21 amplification,p53 deletion,RB1 deletion and IGH rearrangement was lower than that of negative patients. Conclusion The higher the incidence of positive FISH test results,the higher the DS stage and the R-ISS stage. and 1q21 amplification,p53 deletion,RB1 deletion,and IGH rearrangement were independent prognostic factors of MM patients.

关 键 词：多发性骨髓瘤 荧光原位杂交 Durie-Salmon分期 R-ISS分期 相关性 

分 类 号：R733.3[医药卫生—肿瘤]