转录组分析sgf73基因缺失对粟酒裂殖酵母生长代谢影响的分子机制  

Molecular Mechanism of Eeffect of sgf73 Gene Deletion on Growth and Metabolism of Schizosaccharomyces pombe by Transcriptome Analysis

作　　者：刘欣岚 叶姿妤 鲁艳 侯怡铃[2] 周丽倩 蒲帝宏 丁祥 LIU Xinlan;YE Ziyu;LU Yan;HOU Yiling;ZHOU Liqian;PU Dihong;DING Xiang(College of Environmental Science and Engineering,China West Normal University,Nanchong 637009,Sichuan Province,China;Key Laboratory of Southwest China Wildlife Resources Conservation of the Ministry of Education,College of Life Science,China West Normal University,Nanchong 637009,Sichuan Province,China)

机构地区：[1]西华师范大学环境科学与工程学院,四川南充637009 [2]西华师范大学生命科学学院,西南野生动植物资源保护教育部重点实验室,四川南充637009

出　　处：《吉林大学学报（理学版）》2023年第2期426-436,共11页Journal of Jilin University:Science Edition

基　　金：四川省科技厅应用基础重点项目(批准号:2018JY0087);四川省科技厅重点研发项目(批准号:2018NZ0055);南充市科技局发展计划项目(批准号:20YFZJ0053,20YFZJ0054).

摘　　要：为研究粟酒裂殖酵母sgf73基因缺失后的关键基因和关键代谢通路,采用RNA-Seq测序技术对野生型酵母菌株及sgf73基因缺陷型菌株进行测序及生物信息学分析,并进行GO和KEGG功能富集分析.结果表明:sgf73基因缺陷型菌株中高表达基因有1834个,极高表达基因有6个,且有1714个差异表达基因,包括934个上调基因,780个下调基因;sgf73基因敲除导致细胞代谢和跨膜转运过程异常变化;MAPK信号通路中参与周期调控cki1,cki2和cdc25基因的下调导致sgf73Δ菌株有丝分裂时间延长;调控细胞骨架rgf2,rho1和stt4基因的下调导致sgf73Δ菌株肌动蛋白环收缩异常.In order to study the key genes and key metabolic pathways after the sgf 73 gene was knocked out in Schizosaccharomyces pombe,the wild-type yeast strains and sgf73Δgene-deficient strains were sequenced and bioinformatics analyzed by RNA-Seq sequencing technology,and the GO and KEGG functional enrichment analysis were carried out.The results show that in the sgf73Δgene-deficient strains,there are 1834 highly expressed genes,including 6 extremely highly expressed genes,and 1714 differentially expressed genes,of which 934 genes are up-regulated and 780 genes are down-regulated.The sgf73 gene knockout leads to abnormal changes in cellular metabolism and transmembrane transport.The down-regulation of cki1,cki2 and cdc25 genes involved in cycle regulation in the MAPK signaling pathway leads to prolongation of mitotic time in sgf73Δstrain,the down-regulation of regulatory cytoskeleton rgf2,rho1 and stt4 genes leads to abnormal contraction of actin ring of sgf73Δstrain.

关 键 词：粟酒裂殖酵母 RNA-Seq测序 sgf73基因 差异表达基因 MAPK信号通路 

分 类 号：Q343[生物学—遗传学]