人工牛黄通过MEOX2/PI3K/AKT通路调控肾母细胞瘤细胞恶性生物学行为的作用研究  

Artificial Bezoar in Regulating Malignant Biological Behavior of Wilms Tumor Cells Through MEOX2/PI3K/AKT Pathway

作　　者：李正伟[1] 刘奎[1] 王瑛 冯珏利 张大[2] LI Zhengwei;LIU Kui;WANG Ying(The First Affiliated Hospital of Henan University of Science and Technology,Luoyang,471000)

机构地区：[1]河南科技大学第一附属医院,471000 [2]郑州大学第一附属医院,450000

出　　处：《实用癌症杂志》2023年第3期366-370,共5页The Practical Journal of Cancer

基　　金：2019年河南省医学科技攻关项目(编号:LHGJ20190295)。

摘　　要：目的 研究人工牛黄对肾母细胞瘤细胞恶性生物学行为的作用,并探讨相关机制。方法 取对数期SK-NEP-1细胞,将MEOX2-siRNA转染至SK-NEP-1细胞,随机分为转染组、人工牛黄+转染组。转染组加入DMEM培养基常规培养,人工牛黄+转染组加入人工牛黄溶液(10μg/mL)。另取对数期细胞分为对照组和人工牛黄组,对照组加入DMEM培养基常规培养,人工牛黄组加入人工牛黄溶液(10μg/mL)。Western blot检测MEOX2蛋白相对表达量;MTT法检测细胞增殖能力;划痕实验检测细胞迁移能力;Transwell实验检测细胞侵袭能力;Western blot法检测细胞中蛋白激酶B(AKT)、p-AKT、哺乳动物雷帕霉素靶蛋白(mTOR)、p-mTOR蛋白表达。结果 与对照组比较,人工牛黄组MEOX2蛋白表达量升高,24、48、72 h三个时间点A值,迁移率降低,穿膜细胞数/视野减少(P<0.05),转染组MEOX2蛋白表达量降低,24、48、72 h三个时间点A值,迁移率升高,穿膜细胞数/视野增加(P<0.05);与转染组比较,人工牛黄+转染组MEOX2蛋白表达量升高,24、48、72 h三个时间点A值,迁移率降低,穿膜细胞数/视野减少(P<0.05);与对照组比较,人工牛黄组p-AKT/AKT、p-mTOR/mTOR降低(P<0.05),转染组p-AKT/AKT、p-mTOR/mTOR升高(P<0.05);与转染组比较,人工牛黄+转染组p-AKT/AKT、p-mTOR/mTOR降低(P<0.05)。结论 人工牛黄可抑制肾母细胞瘤细胞增殖、侵袭、迁移,其作用机制可能与调控MEOX2/PI3K/AKT通路有关。Objective To study the effect of artificial bezoar on the malignant biological behavior of Wilms tumor cells and to explore the related mechanisms.Methods Take log-phase SK-NEP-1 cells, transfect MEOX2-siRNA into SK-NEP-1 cells, and randomly divide them into transfection group and artificial bezoar + transfection group.The transfection group was added with DMEM medium for routine culture, and the artificial bezoar + transfection group was added with artificial bezoar solution(10 μg/mL).In addition, the log phase cells were divided into the control group and the artificial bezoar group.The control group was routinely cultured with DMEM medium, and the artificial bezoar group was added with artificial bezoar solution(10 μg/mL).Western blot was used to detect the relative expression of MEOX2 protein.MTT method was used to detect cell proliferation ability, Scratch test was used to detect cell migration ability.Transwell experiment was used to detect cell invasion ability.Western blot was used to detect protein kinase B(AKT),p-AKT,mammals in cells Target of rapamycin(mTOR) and p-mTOR protein expression.Results Compared with the control group, the MEOX2 protein expression in the artificial bezoar group was increased, of which the A value at the three time points of 24,48,and 72 h, the migration rate were decreased, of which the number of penetrating cells/field of view was decreased(P<0.05).The expression of MEOX2 protein in the transfection group was decreased, of which the A value at the three time points of 24,48,and 72 h, the migration rate were increased, and the number of transmembrane cells/field of view was increased(P<0.05).Compared with the transfection group, the MEOX2 protein expression in the artificial bezoar + transfection group was increased, of which the A value at the 3 time points of 24,48,and 72 h, the migrationrate were decreased, and the number of penetrating cells/field of view was decreased(P<0.05).Compared with the control group, p-AKT/AKT and p-mTOR/mTOR in the artificial bezoar group were

关 键 词：人工牛黄 肾母细胞瘤 增殖 侵袭 迁移 

分 类 号：R737.11[医药卫生—肿瘤]