蛋白饲料酵母高产菌株的选育及发酵工艺优化研究  

Screening and Breeding of High Yield Strain of Protein Feed Yeast and Optimization of Its Fermentation Process

作　　者：马良[1] 杜泽娅 周翔[1,2] 陈积红 MA Liang;DU Zeya;ZHOU Xiang;CHEN Jihong(Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 73000,China;University of Chinese Academy of Sciences,Beijing 100049,China;Kejin Innovation Institute of Heavy Ion Beam Biological Industry,Baiyin 730900,Gansu,China)

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]中国科学院大学,北京100049 [3]白银市科近重离子束生物产业创新研究院,甘肃白银730900

出　　处：《原子核物理评论》2022年第4期512-518,共7页Nuclear Physics Review

基　　金：内蒙古自治区科技重大专项子课题项目(E039211SZ1)。

摘　　要：饲料中的蛋白含量,是衡量饲料优劣的主要指标,而决定饲料蛋白含量高低的主要因素包括:菌体蛋白含量(酵母菌、乳酸菌等)及饲草中自身蛋白含量;其中饲料酵母菌株蛋白含量直接决定了饲料蛋白的含量,因此获得优良的饲料酵母菌株成为关键。本研究利用辐射能量为80 MeV/u的^(12)C^(6+)重离子束对出发饲料酵母菌种NJ3236(蛋白质量分数为40.64%)进行辐照诱变,并对辐照后的菌种进行初筛、复筛得到高蛋白含量菌株100G-2,该菌株较NJ3236蛋白含量提高了10.08%。利用响应面分析法对发酵培养基进行优化,通过优化得到的培养基的最优配比为:甜高粱汁20.95 g/L、玉米浆干粉18.17 g/L、硫酸镁1.60 g/L,在此条件下可溶性蛋白浓度达到1.381 mg/mL,较未优化前可溶性蛋白提高了8.7%。Protein content in feed is a key index for evaluating feed quality,However,recent reports of determine the protein content of feed on dietary for feeds tuffs investigated as potential sources of protein content(yeast,lactic acid bacteria,etc.)and protein content of the forage.The protein content of feed yeast strains directly determines the protein content of feed,so it becomes the key to obtain good feed yeast strains.In this study,a heavy ion ^(12)C^(6+)beam with an energy of 80 MeV/u was used for irradiation mutagenesis and breeding of starter yeast NJ3236(protein content of 40.64%),and the strains were screened and rescreened to obtain 100G-2 strain with high protein content.The protein content of the 100G-2 strain increased by 10.08%compared with that of the strain NJ3236.Then response surface analysis was used to optimize the fermentation medium.The optimal ratio of medium showed that sweet sorghum juice 20.95 g/L,corn steep liquor 18.17 g/L and magnesium sulfate 1.60 g/L.Under this condition,the soluble protein concentration reached 1.381 mg/mL,which was 8.7%higher than that before optimization.

关 键 词：重离子辐照 饲料酵母 甜高粱汁 响应面法 

分 类 号：Q691[生物学—生物物理学]