人类白细胞抗原G在甲状腺乳头状癌患者外周血和癌组织中的表达  

Expression of HLA-G in the peripheral blood and cancerous tissues of patients with papillary thyroid cancer

作　　者：朱林波 李鹏飞 王敏 刘春姣 李雅丽 李吉 邬一军 Zhu Linbo;Li Pengfei;Wang Min;Liu Chunjiao;Li Yali;Li Ji;Wu Yijun(Department of Thyroid and Breast Surgery,Beilun Branch of the First Affiliated Hospital of Zhejiang University,Ningbo 315800,China;Department of Pathology,Beilun Branch of the First Affiliated Hospital of Zhejiang University,Ningbo 315800,China;Department of Thyroid Surgery,the First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China)

机构地区：[1]浙江大学附属第一医院北仑分院甲乳外科,宁波315800 [2]浙江大学附属第一医院北仑分院病理科,宁波315800 [3]浙江大学附属第一医院甲状腺外科,杭州310003

出　　处：《中华内分泌外科杂志》2023年第1期29-33,共5页Chinese Journal of Endocrine Surgery

基　　金：宁波市自然科学基金项目(202003N4037)。

摘　　要：目的探讨人类白细胞抗原G(human leukocyte antigen-G,HLA-G)在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)患者外周血和癌组织中的表达及其与临床病理特征之间的关系。方法采用酶联免疫吸附实验(ELISA)法检测浙江大学附属第一医院北仑分院甲乳外科50例PTC患者(PTC组)、25例甲状腺良性肿瘤患者(结节性甲状腺肿19例和甲状腺腺瘤6例,即甲状腺良性肿瘤组)和浙江大学附属第一医院北仑分院健康体检中心20例正常体检人群(健康对照组)外周血中可溶性HLA-G(sHLA-G)的表达量。采用免疫组织化学(EnVision)法检测前述PTC组织、甲状腺良性肿瘤组织中HLA-G的表达水平,并分析其与甲状腺癌临床病理参数间的关系。采用SPSS 19.0进行统计分析,正态分布计量资料用两独立样本t检验,两组间率的比较采用χ^(2)检验。结果手术前PTC组、甲状腺良性肿瘤组和健康对照组患者外周血中sHLA-G的表达量分别为(21.33±5.54)、(22.73±4.99)和(18.29±4.43)ng/ml。与健康对照组相比,PTC组、甲状腺良性肿瘤组sHLA-G水平均明显增高,差异具有统计学意义[PTC组vs健康对照组(t=2.18,P=0.032);甲状腺良性肿瘤组vs健康对照组(t=3.12,P=0.003)]。PTC组和甲状腺良性肿瘤组sHLA-G水平比较差异无统计学意义(t=1.07,P=0.289)。HLA-G在PTC组织中的阳性表达率为78%(39/50),其中弱阳性为38%(19/50),中度阳性为26%(13/50),强阳性为14%(7/50)。PTC癌旁正常组织中未见HLA-G表达。HLA-G在结节性甲状腺肿、甲状腺腺瘤等良性肿瘤中均不表达。HLA-G与PTC肿瘤最大径有关,最大径>1 cm的阳性表达率明显高于直径≤1 cm的阳性表达率(P<0.05)。HLA-G阳性表达率与PTC患者的性别、年龄、多发病灶、腺外侵犯、淋巴结转移和TNM分期无明显相关(χ^(2)=4.45,P=0.035)。结论HLA-G在PTC癌组织中显著高表达,与PTC肿瘤直径相关,可能在PTC中发挥重要作用。外周血sHLA-G可能与甲状腺肿瘤的形�Objective To examine the expression of human leukocyte antigen G(HLA-G)in the peripheral blood and cancerous tissues of patients with papillary thyroid carcinoma(PTC).Methods The expression of soluble HLA-G(sHLA-G)in the peripheral blood of 50 individuals with PTC(PTC group),25 patients with benign thyroid tumors(BTT group)from Department of Thyroid and Breast Surgery,Beilun branch of the First Affiliated Hospital of Zhejiang University and 20 healthy controls(healthy control group)from physical examination center was assessed by ELISA.Immunohistochemical examination of HLA-G levels was also performed on tissue specimens from patients in the PTC and BTT groups,and their correlation with clinicopathological features of thyroid cancer was analyzed.SPSS 19.0 was used for statistical analysis.The measurement data of normal distribution were tested by two independent samples t test.Chi square test was used to compare the rates between the two groups.Results The sHLA-G expression in peripheral blood was 21.33(±5.54),22.73(±4.99),and 18.29(±4.43)ng/mL in the preoperative PTC,BTT,and healthy control groups,respectively.Compared to the healthy group,sHLA-G levels were considerably higher in the PTC and BTT groups,with statistically significant differences(totally P<0.05).There was no significant difference in statistically sHLA-G levels between the BTT and PTC groups(P>0.05).The positive HLA-G expression rate in PTC tissues was 78%(39/50).There was no evidence of HLA-G expression in common tissues adjacent to PTC.HLA-G was not expressed in benign tumors.HLA-G was linked with the PTC tumor diameter,and the rate of positive expression was considerably greater with tumor diameters>1 cm than with those≤1 cm(P<0.05).The rate of HLA-G positive expression was not significantly correlated with sex,age,multiple foci,extra-glandular invasion,metastasis of lymph nodes,or the TNM stage in PTC individuals(P>0.05).Conclusions HLA-G is significantly expressed at high levels in PTC tissues,is correlated with the tumor diameter,and m

关 键 词：甲状腺乳头状癌 人类白细胞抗原G 肿瘤标记物 

分 类 号：R736.1[医药卫生—肿瘤]