结肠癌组织中SRSF1蛋白的表达及其临床意义  

Expression and clinical significance of SRSF1 in colon cancers

作　　者：王姝昊 常肖璐 赵磊[2] 孙紫洋 朴俊杰[2] 林贞花[2] 金爱花 WANG Shu-hao;CHANG Xiao-lu;ZHAO Lei;SUN Zi-yang;PIAO Jun-jie;LIN Zhen-hua;JIN Ai-hua(Department of Gastroenterology,Yanbian University Affiliated Hospital,Yanji 133002,China;Key Laboratory of Pathobiology/Yanbian University,State Ethnic Affairs Commission,Yanji 133002,China)

机构地区：[1]延边大学附属医院消化内科,延吉133002 [2]民族地区高发肿瘤病理生物学国家民委重点实验室(延边大学),延吉133002

出　　处：《临床与实验病理学杂志》2023年第2期146-151,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：吉林省教育厅科学技术研究项目(JJKH20220545KJ)。

摘　　要：目的 探讨SRSF1在结肠癌中的表达及其与临床病理特征、预后的关系。方法 应用癌症基因组图谱(TCGA)和GEO数据库分析SRSF1 mRNA在结肠癌、正常结肠组织中的表达。采用免疫组化法检测SRSF1蛋白在94例结肠癌和86例癌旁正常组织中的表达,分析其表达与结肠癌临床病理特征的关系。应用Kaplan-Meier生存法分析SRSF1蛋白表达与结肠癌患者生存时间的相关性;采用Cox回归模型分析SRSF1在结肠癌风险预测中的评估价值。结果 TCGA和GEO数据库结果显示SRSF1 mRNA在结肠癌中高表达(P<0.05)。免疫组化检测显示SRSF1蛋白在结肠癌组织中的阳性率和高表达率分别为92.6%(87/94)和67.0%(63/94),显著高于癌旁正常组织(57.0%、11.6%)。χ^(2)=检验结果显示SRSF1表达水平与结肠癌患者临床分期相关(P=0.028)。Kaplan-Meier生存分析表明:SRSF1蛋白高表达结肠癌患者的总生存期低于SRSF1蛋白低表达患者(P=0.040)。Cox单因素回归分析表明:淋巴结转移(HR:3.638,95%CI=1.913~6.917,P<0.001)、临床肿瘤分期(HR:4.313,95%CI=2.228~8.348,P<0.001)和SRSF1蛋白高表达(HR:2.130,95%CI=1.010~4.492,P=0.047)是影响结肠癌患者预后的独立风险因素。结论 SRSF1表达与结肠癌的发生、发展及预后密切相关,可作为结肠癌预后评估的潜在标志物。Purpose To investigate the expression of SRSF1 in colon cancer and its relationship with clinical features and prognosis. Methods The mRNA expression of SRSF1 in normal and colon cancer tissues were analyzed by using TCGA and GEO databases. Immunohistochemical(IHC) staining was performed to detect the expression of SRSF1 protein in 94 colon cancer tissues and 86 paraneoplastic non-tumor tissues. The relationship between SRSF1 expression and pathological parameters were analyzed. Additionally, the correlation between SRSF1 and survival of colon cancer patients was determined by Kaplan-Meier analysis. Moreover, the prognostic value of SRSF1 in colon cancer was evaluated by Cox-regression model. Results TCGA and GEO databases showed that SRSF1 expression was significantly up-regulated in colon cancers(P<0.05). IHC staining showed that the positive rate of SRSF1 in colon cancer tissues(92.6%, 87/94 and 67.0%, 63/94) was significantly higher than that in paraneoplastic non-tumor tissues(57.0% and 11.6%, P<0.05). Chi-square test showed that the expression of SRSF1 was related to clinical stages(P=0.028). Kaplan-Meier survival analysis showed that high expression of SRSF1 was related to shorter survival time. Cox regression analysis showed that lymph node metastasis(HR=3.638, 95%CI= 1. 913-6. 917,P < 0. 001),clinical tumor stage(HR =4. 313,95% CI = 2. 228-8. 348,P < 0. 001) and SRSF1 protein expression(HR = 2. 130,95% CI = 1. 010-4. 492,P =0. 047) were independent risk factors for colon cancer. Conclusion Elevated expression of SRSF1 is closely related to the progression and prognosis of colon cancers. SRSF1 may potentially be used as an important prognostic indicator for colon cancer.

关 键 词：结肠肿瘤 SRSF1 免疫组织化学 生存分析 预后 

分 类 号：R735.3[医药卫生—肿瘤]