Idylla^(TM)全自动PCR法在非小细胞肺癌FFPE组织EGFR检测中的应用  

Application of Idylla^(TM) automatic PCR method in the detection of EGFR in FFPE tissues of non-small cell lung cancer

作　　者：杨长绍 潘鑫艳 李光芬 韩敏 付思琪 李涛 YANG Chang-shao;PAN Xin-yan;LI Guang-fen;HAN Min;FU Si-qi;LI Tao(Department of Pathology,Yunnan New Kunhua Hospital,Kunming 650301,China;Department of Pathology,Lufeng People's Hospital,Lufeng 651200,China;Department of Pathology,920 Hospital of Joint Logistics Support Force,Kunming 650032,China)

机构地区：[1]云南新昆华医院病理科,昆明650301 [2]禄丰市人民医院病理科,禄丰651200 [3]解放军联勤保障部队第九二〇医院病理科,昆明650032

出　　处：《诊断病理学杂志》2022年第11期996-1000,共5页Chinese Journal of Diagnostic Pathology

摘　　要：目的 探讨Idylla^(TM)全自动PCR法在非小细胞肺癌(non-small cell lung cancer, NSCLC)患者石蜡切片表皮因子生长受体(epidermal growth factor receptor, EGFR)基因检测中的应用,并分析与临床病理特征的相关性。方法 选取116例NSCLC患者,其中手术标本85例,穿刺标本11例,恶性胸腔积液细胞块20例。运用Idylla^(TM)全自动PCR法检测116例NSCLC患者EGFR基因18、19、20、21外显子突变情况,且采用直接测序法对30例手术标本、11例穿刺标本及20例细胞块进行验证,并统计分析各类突变与患者临床病理特征的相关性。结果 116例NSCLC患者EGFR基因总突变率为44.0%(51/116),其中EGFR18 G719X突变占3.9%(2/51),EGFR19缺失突变占39.2%(20/51),EGFR21 L858R突变占41.2%(21/51),5例样本9.8%(5/51)存在G719X、L861Q双突变,2例样本3.9%(2/51)存在19Del、T790M双突变,1例样本2.0%(1/51)存在19Del、L858R双突变。EGFR基因突变与患者性别、组织学类型及是否吸烟相关(P<0.05),而与患者样本类型及是否转移无明显相关(P>0.05)。结论 Idylla^(TM)全自动PCR法具有操作简单、自动化强、样本用量少等优势,可应用于不同样本类型的FFPE组织EGFR检测,且结果可靠。Objective To explore the application of Idylla^(TM) automatic PCR method in the detection of the epidermal growth factor receptor(EGFR) gene in paraffin sections of patients with non-small cell lung cancer(NSCLC), and to analyze the correlation of EGFR gene mutation with clinicopathological characteristics. Methods A total of 116 cases of NSCLC patients were collected, including 85 cases of surgical specimens, 11 cases of puncture specimens, and 20 cases of malignant pleural effusion cell blocks. The mutations of EGFR gene exons 18, 19, 20, and 21 in 116 patients with NSCLC were detected by the Idylla^(TM) fully automated PCR method. The 30 cases of surgical specimens, 11 cases of puncture specimens and 20 cases of cell blocks were verified by the direct sequencing method. Then the various mutations and their relevance to the clinicopathologic characteristics were statistically analyzed. Results The total mutation rate of EGFR gene in 116 cases of NSCLC were 44.0%(51/116), in which EGFR18 G719 X accounted for 3.9%(2/51), EGFR19 Del accounted for 39.2%(20/51), and EGFR21 L858 R accounted for 41.2%(21/51). G719 X and L861 R double mutations were found in 5 cases(9.8%) of the patients, 19 Del and T790 M double mutations were found in 2 cases(3.9%). We also found that there were 19 Del and L858 R double mutations in one sample. The EGFR gene mutation was related to the patient’s gender, histological type and smoking status(P<0.05), but not significantly related to sample type and lymph node metastasis(P>0.05). Conclusion Idylla^(TM) automated PCR method has the advantages of simple operation, strong automation, and less sample. It can be applied to EGFR detection of FFPE tissues of different sample types, and the results are reliable.

关 键 词：Idylla^(TM)全自动PCR法 非小细胞肺癌 FFPE组织 表皮因子生长受体 

分 类 号：R734.2[医药卫生—肿瘤]