烟草幼苗应答低温胁迫的转录组和蛋白质组分析  

Analysis of transcriptome and proteome of tobacco seedlings responding to low temperature stress

作　　者：王国平 齐胤尧 许杰 索文龙 徐国云[3] 刘婷婷 牛永志 乔雨 郑昀晔 WANG Guoping;QI Yinyao;XU Jie;SUO Wenlong;XU Guoyun;LIU Tingting;NIU Yongzhi;QIAO Yu;ZHENG Yunye(Yuxi Zhongyan Tobacco Seed Co.,Ltd,Yuxi 653100,Yunnan,China;College of Biology,Hunan University,Changsha 410082,Hunan,China;Zhengzhou Tobacco Research Institute of China National Tobacco Corporation,Zhengzhou 450001,Henan,China)

机构地区：[1]玉溪中烟种子有限责任公司,云南玉溪653100 [2]湖南大学生物学院,湖南长沙410082 [3]中国烟草总公司郑州烟草研究院,河南郑州450001

出　　处：《中国烟草学报》2023年第1期71-78,共8页Acta Tabacaria Sinica

基　　金：中国烟草总公司云南省公司科技计划一般项目“多肽激素调控烟草种子活力研究与应用探索”(2021530000242033)。

摘　　要：【背景和目的】低温是烟草育苗过程中遇到的主要逆境之一,对培育适龄健壮的幼苗具有不利影响,为探究烟草应答低温胁迫的分子机理。【方法】将烟草幼苗进行低温胁迫处理不同时间,通过转录组、蛋白组联合分析低温胁迫下的应答事件。【结果】随着低温处理时间的增加,应答低温胁迫的基因数目不断增加。低温处理下,差异表达基因主要富集到与磷酸化、乙烯途径以及光合作用相关的信号通路,而差异表达蛋白也同时富集到与光合作用相关通路。此外,本研究鉴定到5种参与低温应答的多肽,其中低温处理12 h后的Nitab4.5_0000037g0360多肽在转录组与蛋白组中同时被鉴定到,可能在低温应答过程中起重要作用。【结论】本研究挖掘了烟草幼苗应答低温的基因和蛋白,并为后续挖掘多肽在低温应答过程中的功能提供了理论基础。Low temperature is one of the major stresses during tobacco seedlings process, which has negative effects on the cultivation of robust tobacco seedlings with appropriate age. In order to analyze how tobacco responds to low temperature stress, tobacco seedlings were treated with low temperature for different treatment time, and the response events were analyzed through conjoint analysis based on transcriptome and proteome. The number of genes responding to low temperature stress increased with the extension of treatment time.Under low temperature treatment, the major enriched signaling pathways of differentially expressed genes were related to phosphorylation,ethylene pathway and photosynthesis, and the major enriched signaling pathways of differentially expressed proteins were also related to photosynthesis. In addition, six peptides involved in low temperature stress response were identified, where Nitab4.5_0000037g0360 polypeptide was identified in both transcriptome and proteome after 12 h of low temperature treatment, suggesting that it may play an important role in the low temperature response. This study reveals the genes and proteins of tobacco seeding responding to low temperature stress, which provides a theoretical basis for the subsequent exploration of the function of peptides in the low temperature response process.

关 键 词：转录组 蛋白组 烟草幼苗 低温 多肽 

分 类 号：S572[农业科学—烟草工业]