METTL3和ALKBH5在甲状腺癌中的表达  

Expression of METTL3 and ALKBH5 in thyroid carcinoma

作　　者：谭屾[1] 崔爱民[1] 柏楠[1] 查晔军[2] 卢帅 TAN Shen;CUI Aimin;BAI Nan;ZHA Yejun;LU Shuai(Department of General Surgery,Beijing Jishuitan Hospital,Beijing,China,100000;Department of Orthopaedic,Beijing Jishuitan Hospital,Beijing,China,100000)

机构地区：[1]北京积水潭医院普外科,北京100000 [2]北京积水潭医院骨科,北京100000

出　　处：《分子诊断与治疗杂志》2023年第2期185-188,196,共5页Journal of Molecular Diagnostics and Therapy

基　　金：北京市自然科学基金-海淀原始创新联合基金资助项目(L192049)。

摘　　要：目的 探讨甲基转移酶样3(METTL3)和alkB同系物5(ALKBH5)在甲状腺癌(TC)中的水平及意义。方法 选取2014年3月至2015年9月于北京积水潭医院住院治疗的116例TC患者癌组织作为TC组,选取其相应癌旁组织作为对照组。检测组织METTL3、ALKBH5 mRNA水平;分析METTL3、ALKBH5 mRNA水平与患者临床病理特征的关系;分析METTL3 mRNA与ALKBH5 mRNA表达的相关性;以Kaplan-Meier法分析TC组织中METTL3、ALKBH5表达与患者五年内总生存率的关系;采用多因素Cox回归分析TC预后的影响因素。结果 TC组METTL3 mRNA水平高于对照组,ALKBH5mRNA水平低于对照组,差异均有统计学意义(P<0.05)。METTL3高表达组患者TNMⅢ期、淋巴结转移比例高于低表达组,ALKBH5低表达组TNMⅢ期、淋巴结转移比例高于高表达组,差异均有统计学意义(P<0.05)。TC组织中METTL3 m RNA与ALKBH5 mRNA表达水平呈负相关(r=-0.589,P<0.05)。METTL3高表达组五年总生存率低于METTL3低表达组,ALKBH5低表达组五年内总生存率低于ALKBH5高表达组,差异均有统计学意义(P<0.05)。TNM分期Ⅲ期、淋巴结转移及METTL3高表达、ALKBH5低表达是影响TC预后的独立危险因素(P<0.05)。结论 TC中METTL3呈高表达、ALKBH5呈低表达,均可作为TC患者的独立预后因素。Objective To investigate the levels and significance of methyltransferase like 3(METTL3)and alkB homolog 5(ALKBH5)in thyroid carcinoma(TC). Methods The cancer tissues of 116TC patients hospitalized in Beijing Jishuitan Hospital from March 2014 to September 2015 were selected as the TC group,and the corresponding adjacent tissues were selected as the control group. The levels of METTL3 and ALKBH5 mRNA were detected;the relationship between the levels of METTL3,ALKBH5 mRNA and the clinicopathological features of the patients was analyzed. The correlation between METTL3 mRNA and ALKBH5 mRNA expression was analyzed. The relationship between the expression of METTL3 and ALKBH5 in TC tissues and the five-year overall survival rate was analyzed by Kaplan-Meier method. Multivariate Cox regression was used to analyze the factors influencing the prognosis of TC. Results The level of METTL3mRNA in the TC group was higher than that in the control group,and the level of ALKBH5 mRNA in the TC group was lower than that in the control group(P<0.05). The proportion of TNM stage Ⅲ and lymph node metastasis in the METTL3 high expression group was higher than that in the low expression group,and the proportion of TNM stage Ⅲ and lymph node metastasis in the ALKBH5 low expression group was higher than that in the high expression group(P<0.05). The expression level of METTL3 mRNA and ALKBH5 mRNA in TC tissues was negatively correlated(r=-0.589,P<0.05). The five-year overall survival rate of the METTL3high expression group was lower than that of the METTL3 low expression group,and the five-year overall survival rate of the ALKBH5 low expression group was lower than that of the ALKBH5 high expression group,and the differences were statistically significant(P<0.05). TNM stage Ⅲ,lymph node metastasis,high expression of METTL3 and low expression of ALKBH5 were independent risk factors affecting the prognosis of TC(P<0.05). Conclusion The high expression of METTL3 and the low expression of ALKBH5 in TC can be used as independent progn

关 键 词：甲状腺癌 甲基转移酶样3 AlkB同系物5 

分 类 号：R736.1[医药卫生—肿瘤]