改良冷冻组织方法对术中切片质量及诊断的意义  被引量:2

Significance of improved tissue freezing method for intraoperative section quality and diagnosis

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作  者:刘菲菲[1] 李雪[1] LIU Fei-fei;LI Xue(Department of Pathology,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020 China)

机构地区:[1]首都医科大学附属北京朝阳医院病理科,北京100020

出  处:《诊断病理学杂志》2022年第12期1111-1114,1119,共5页Chinese Journal of Diagnostic Pathology

摘  要:目的 探讨冷冻组织方法对术中冷冻切片质量及诊断的影响。方法 选取2019-09—2021-10符合入组条件的2070例冷冻标本,分别使用传统法、模具法和改良法冷冻组织,分析冷冻方法对切片质量及诊断的影响。结果 改良法质量评分最高15.39,优片数(评分≥14)621张,传统法14.73(528张),模具法14.44(412张),切片质量评分的差异有统计学意义(P<0.001),无冰晶、裂隙、褶皱,厚薄均匀及组织结构清晰片数的差异有统计学意义(P<0.01)。改良法无冰晶片数最高628张,传统法540张、模具法421张,差异有统计学意义(P<0.01),淋巴结、甲状腺、肺组织易产生冰晶,改良法减少冰晶产生优于其他方法(P<0.001),冷冻时间和返工率方面也占明显优势[(30±5)s, 1.88%]。结论 改良冷冻组织方法能有效改善术中切片质量,加快制片速度并降低返工率,保证了术中病理诊断结果准确性并减少用时。Objective To investigate the influence of frozen tissue method on the quality and diagnosis of intraoperative frozen sections. Methods A total of 2070 frozen specimens were selected and divided into three groups from September 2019 to October 2021. The tissues were frozen by traditional method, mold method and improved method, respectively, and the effects of freezing method on the quality of sections and diagnosis were analyzed. Results The quality score of the improved method was the highest 15.39, with 621 excellent slices under microscope(score ≥ 14), followed by 14.73 and 528 by the traditional method, 14.44 and 412 by the mold method. There were significant differences in slice quality scores(P<0.001);no ice crystals, cracks and folds, uniform thickness and clear tissue structure were also significant differences in slice numbers(P<0.01). The improved method had the highest number of ice-free slices(628), followed by the traditional method(540), and mold method(421), with significant difference(P<0.01). Ice crystals were easily produced in lymph nodes, thyroid gland and lung tissues. The improved method was superior to other methods in inhibiting ice crystal production(P<0.001), and had obvious advantages in freezing time and rework rate(30±5 s, 1.88%). Conclusion The improved frozen tissue method could effectively improve the quality of intraoperative section, speed up the slice making and reduce the rework rate, ensure the accuracy of intraoperative pathological diagnosis and improve the timeliness of diagnosis.

关 键 词:冷冻组织 切片质量 冰晶 术中病理诊断 

分 类 号:R446.8[医药卫生—诊断学]

 

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