circAGFG1在皮肤鳞状细胞癌组织中的表达及其对SCC13细胞增殖、迁移的影响  

Expression of circAGFG1 in cutaneous squamous cell carcinoma and its effect on proliferation and migration of SCC13 cells

作　　者：宋丹丹[1] 宋明旭[2] 孙鸿[1] SONG Dandan;SONG Mingxu;SUN Hong(Department of Dermatology,Affiliated Hospital of Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学附属医院皮肤科,无锡214122 [2]江南大学附属医院肿瘤研究所,无锡214122

出　　处：《中国免疫学杂志》2023年第2期359-363,共5页Chinese Journal of Immunology

摘　　要：目的:探讨circAGFG1在皮肤鳞状细胞癌组织中的表达及其对SCC13细胞增殖、迁移的影响及其可能作用机制。方法:收集2018年3月至2020年5月江南大学附属医院收治的46例皮肤鳞状细胞癌患者的癌组织及其相应癌旁组织标本,qRT-PCR法检测circAGFG1、miR-653-5p的表达量;采用Pearson法分析皮肤鳞状细胞癌组织中circAGFG1与miR-653-5p表达量的相关性;体外培养人皮肤鳞状细胞癌细胞SCC13,随机分为:si-NC组、si-circAGFG1组、miR-NC组、miR-653-5p组、si-circ-AGFG1+anti-miR-NC组、si-circAGFG1+anti-miR-653-5p组;CCK-8法、平板克隆形成实验、划痕实验与Transwell实验分别检测细胞增殖、克隆形成及迁移;双荧光素酶报告实验检测circAGFG1与miR-653-5p的靶向关系。结果:与癌旁组织比较,皮肤鳞状细胞癌组织中circAGFG1的表达量升高(P<0.05),miR-653-5p的表达量降低(P<0.05);circAGFG1与miR-653-5p呈负相关(r=−0.5717,P<0.001);与si-NC组比较,si-circAGFG1组miR-653-5p的表达量升高(P<0.05),细胞增殖抑制率升高(P<0.05),划痕愈合率降低(P<0.05),细胞克隆形成数和迁移细胞数减少(P<0.05);circAGFG1可靶向调控miR-653-5p的表达;与miR-NC组比较,miR-653-5p组细胞增殖抑制率升高(P<0.05),划痕愈合率降低(P<0.05),细胞克隆形成数和迁移细胞数减少(P<0.05);与si-circAGFG1+anti-miR-NC组比较,si-circAGFG1+anti-miR-653-5p组细胞增殖抑制率降低(P<0.05),划痕愈合率升高(P<0.05),细胞克隆形成数和迁移细胞数增多(P<0.05)。结论:下调circAGFG1表达可通过靶向调控miR-653-5p表达而抑制皮肤鳞状细胞癌细胞增殖、克隆形成及迁移。Objective:To explore the expression of circAGFG1 in cutaneous squamous cell carcinoma and its effect on the proliferation and migration of SCC13 cells and its possible mechanism.Methods:From March 2018 to May 2020,cancer tissues and corresponding adjacent tissue specimens from 46 patients with cutaneous squamous cell carcinoma admitted to Affiliated Hospital of Jiangnan University were collected,and the expressions of circAGFG1 and miR-653-5p were detected by qRT-PCR.Pearson method was used to analyze the correlation between circAGFG1 and miR-653-5p expressions in cutaneous squamous cell carcinoma tissues.Cultured human cutaneous squamous cell carcinoma cells SCC13 in vitro,randomly grouped:si-NC group,si-circAGFG1 group,miRNC group,miR-653-5p group,si-circAGFG1+anti-miR-NC group,si-circAGFG1+anti-miR-653-5p group.CCK-8 method,plate clone formation test,scratch test and Transwell test were used to detect cell proliferation,clone formation and migration.The dual luciferase reporter experiment was used to detect the targeting relationship between circAGFG1 and miR-653-5p.Results:Compared with adjacent tissues,the expression of circAGFG1 in cutaneous squamous cell carcinoma tissue was increased(P<0.05),while the expression of miR-653-5p was decreased(P<0.05).circAGFG1 was negatively correlated with miR-653-5p(r=−0.5717,P<0.001).Compared with si-NC group,the expression of miR-653-5p in si-circAGFG1 group was increased(P<0.05),the cell proliferation inhibition rate was increased(P<0.05),and the scratch healing rate was decreased(P<0.05),the number of cell clone formation and the number of migrating cells were decreased(P<0.05).circAGFG1 could target the expression of miR-653-5p.Compared with miR-NC group,the cell proliferation inhibition rate in miR-653-5p group was increased(P<0.05),while the scratch healing rate was decreased(P<0.05),and the number of cell clones and the number of migrating cells were decreased(P<0.05).Compared with si-circAGFG1+anti-miR-NC group,the cell proliferation inhibition rate in si-circAG

关 键 词：皮肤鳞状细胞癌 circAGFG1 miR-653-5p 细胞增殖 迁移 

分 类 号：R730.1[医药卫生—肿瘤]