LINC01980在宫颈癌中的表达及调控LIN28B表达促进宫颈癌生长的作用机制  

Expression of LINC01980 in cervical cancer and mechanism of regulating expression of LIN28B to promote growth of cervical cancer

作　　者：王春梅[1] 吴德慧[2] 康燕[1] 魏顺英[3] 马登云[4] WANG Chunmei;WU Dehui;KANG Yan;WEI Shunying;MA Dengyun(Department of Gynecology,Qinghai Red Cross Hospital,Xining 810000,China)

机构地区：[1]青海红十字医院妇一科,西宁810000 [2]青海红十字医院妇五科,西宁810000 [3]青海红十字医院妇二科,西宁810000 [4]青海红十字医院病理科,西宁810000

出　　处：《中国免疫学杂志》2023年第2期369-374,共6页Chinese Journal of Immunology

基　　金：青海省卫生健康委员会指导性计划课题项目(2020-wjzdx-62)。

摘　　要：目的:探讨长链非编码RNA01980(LINC01980)在宫颈癌中的表达及其调控LIN28B促进宫颈癌生长的作用机制。方法:生物信息学工具iBio Tools V5.0(http://www.chrisapp.xyz:3838/R/AnnoE2/)预测宫颈癌差异表达基因,catRAPID数据库(http://service.tartaglialab.com/page/catrapid_group)在线预测相关RNA结合蛋白。选取2020年1月至2021年5月青海红十字医院收治的病理学诊断为宫颈癌的患者60例作为宫颈癌组,选取同期健康体检者(宫颈未发生病变)60例作为健康组,qRTPCR检测两组研究对象血清LINC01980水平;体外培养宫颈癌细胞Hela,转染并分为Lnc-NC组、si-LINC01980组、si-LINC01980+LIN28B-NC组和si-LINC01980+LIN28B组,对照组仅添加新鲜培养液。RNA pull-down和RNA免疫共沉淀试验检测LINC01980与LIN28B相互作用;qRT-PCR检测Hela细胞LINC01980、LIN28B mRNA表达;CCK-8检测细胞活力;流式细胞术检测细胞周期和凋亡;Western blot检测细胞周期蛋白D1(CyclinD1)、周期蛋白依赖性激酶4(CDK4)、半胱氨酸天冬氨酸蛋白酶3(caspase-3)表达。结果:生物信息学工具iBio Tools V5.0预测结果表明,LINC01980在宫颈癌组织中表达上调;与健康组比较,宫颈癌组血清LINC01980 mRNA表达显著升高(P<0.05);生物信息学网站(http://service.tartaglialab.com/page/catrapid_group)预测结果表明,LINC 01980 RNA序列与LIN28存在结合位点,LINC01980与LIN28B存在相互作用;与对照组比较,si-LINC01980组Hela细胞LINC01980、LIN28B mRNA表达、细胞活力、S期、G2/M期细胞百分比、蛋白CyclinD1、CDK4、LIN28B表达显著降低,细胞凋亡率、G0/G1期细胞百分比、蛋白caspase-3表达显著升高(P<0.05);与si-LINC01980组比较,si-LINC01980+LIN28B组Hela细胞活力、S期、G2/M期期细胞百分比、蛋白CyclinD1、CDK4、LIN28B表达显著升高,细胞凋亡率、G0/G1期细胞百分比、caspase-3蛋白表达显著降低(P<0.05)。结论:LINC01980可能通过调控LIN28B影响宫颈癌细胞增殖和凋亡,参与宫颈癌�Objective:To investigate expression of long non-coding RNA01980(LINC01980)in cervical cancer and its mechanism of regulating LIN28B to promote growth of cervical cancer.Methods:Bioinformatics tool iBio Tools V5.0(http://www.chrisapp.xyz:3838/R/AnnoE2/)was used to predict differentially expressed genes in cervical cancer,and catRAPID database(http://service.tartaglialab.com/page/catrapid_group)was used to predict related RNA binding proteins online.Sixty patients with cervical cancer diagnosed as cervical cancer in Qinghai Red Cross Hospital from January 2020 to May 2021 were selected as cervical cancer group,at the same time,another 60 patients with no cervical lesions in health checkup were selected as healthy group.qRT-PCR was used to detect serum LINC01980 level of subjects in two groups;cervical cancer cells Hela were cultured in vitro and divided into Lnc-NC group,si-LINC01980 group,si-LINC01980+LIN28B-NC group and si-LINC01980+LIN28B group by transfection,control group was only added fresh culture medium.RNA pull-down and RNA immunoprecipitation assay were used to detect interaction between LINC01980 and LIN28B.qRT-PCR was used to detect expressions of LINC01980 and LIN28B mRNA in Hela cells;CCK-8 was used to detect cell viability;flow cytometry was used to detect cell cycle and apoptosis;Western blot was applied to detect expressions of cyclinD1(CyclinD1),cyclin-dependent kinase 4(CDK4),and caspase-3.Results:Bioinformatics tool iBio Tools V5.0 prediction results showed that expression of LINC01980 was up-regulated in cervical cancer tissues,compared with healthy group,serum LINC01980 mRNA expression in cervical cancer group was significantly increased(P<0.05);bioinformatics website(http://service.tartaglialab.com/page/catrapid_group)prediction results showed that LINC01980 RNA sequence had a binding site with LIN28;LINC01980 interacted with LIN28B;compared with control group,LINC01980,LIN28B mRNA expressions,cell viability,S phase and G2/M phase cell percentages,CyclinD1,CDK4,LIN28B protein expressions of

关 键 词：长链非编码RNA 01980 LIN28B 宫颈癌 表达 生长 

分 类 号：R737.33[医药卫生—肿瘤]