Pharmacokinetics of Enrofloxacin and Its Metabolite in Carp (Cyprinus carpio) After a Single Oral Administration in Medicated Feed  

作　　者：LIU Ying LI Zhaoxin ZHANG Dahai XING Lihong SUN Weihong SUN Xiaojie PENG Jixing ZHANG Yonggang LI Xianguo 

机构地区：[1]College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China [2]Qingdao Fishery Technology Service Station,Qingdao 266071,China [3]Qingdao Marine Management Support Center,Qingdao 266071,China [4]Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Laboratory of Quality&Safety Risk Assessment for Aquatic Products,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China

出　　处：《Journal of Ocean University of China》2023年第1期171-180,共10页中国海洋大学学报（英文版）

基　　金：supported by the Central Public-Interest Scientific Institution Basal Research Fund,CAFS(No.2020TD71).

摘　　要：A precise and reliable analytical method of high performance liquid chromatography-tandem mass spectrometry(HPLCMS/MS)was developed to measure trace levels of enrofloxacin(ENR)and its major metabolite ciprofloxacin(CIP)in carp tissues.Optimized chromatographic separation was obtained on a Waters Xterra MS C_(18) reversed-phase column using gradient elution with methanol and 0.1%formic acid aqueous solution including 5mmolL^(-1) of ammonium acetate.The established method was applied to study the pharmacokinetics and distribution of ENR and CIP in tissues of carp following a single oral administration in feed at a dosage of 40mgkg^(-1) bw(body weight).Data were analyzed using DAS 2.0 dynamics software,and the experimental results suggest that ENR was rapidly absorbed and extensively distributed in carp tissues through systemic circulation,and the pharmacokinetic characteristics can be described with a two-compartment model.The elimination half-lives(t_(1/2β))from muscle,liver,gill,plasma and skin were 131,160,104,132 and 310 h,respectively.The areas under the drug concentration-time curves(AUC)for these tissues were 491,972,750,249 and 706hmgkg^(-1),respectively.The maximum concentration(C_(max))values were 13,29,37,9 and 5mgkg^(-1) with peak times(t_(max))of 8,4,4,2 and 4 h,respectively.Ciprofloxacin,the active metabolite of ENR,was also detected in carp tissues,indicating that only 1.54%of de-ethylation of ENR occurs in carp.At a water temperature of 18℃,the drug withdrawal time was determined to be no less than 24 d while the carp was fed at a single dosage of 40mgkg^(-1).

关 键 词：Cyprinus carpio ENROFLOXACIN CIPROFLOXACIN PHARMACOKINETICS liquid chromatography-tandem mass spectrometry 

分 类 号：S948[农业科学—水产养殖]