云南省西部地区5岁以下腹泻儿童感染致病性小肠结肠炎耶尔森菌调查  

A investigation of pathogenic Yersinia enterocolitica infection in children with diarrhea under 5 years of age in western Yunnan

作　　者：段彪[1] 郑勇 秦帅 段然[3] 洪梅[1] 艾志琼 肖萌 王鑫[3] DUAN Biao;ZHENG Yong;QIN Shuai;DUAN Ran;HONG Mei;AI Zhi-qiong;XIAO Meng;WANG Xin(Yunnan Institute of Endemic Diseases Control and Prevention,Dali,Yunnan 671000,China;The First Affiliated Hospital of Dali University,Dali,Yunnan 671000,China;National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Dali University,Dali,Yunnan 671000,China)

机构地区：[1]云南省地方病防治所,云南大理671000 [2]大理大学第一附属医院,云南大理671000 [3]中国疾病预防控制中心传染病预防控制所,北京102206 [4]大理大学,云南大理671000

出　　处：《中国热带医学》2023年第2期136-138,156,共4页China Tropical Medicine

基　　金：云南省医疗卫生单位内设研究机构科研项目(No.2018NS0214);徐建国院士工作站研究计划项目(No.2019YS06)。

摘　　要：目的了解云南西部地区≤5岁腹泻儿童小肠结肠炎耶尔森菌的感染水平及病原学特征,为儿童感染性腹泻防治提供依据。方法采集2020—2021年在大理大学第一附属医院就诊的≤5岁腹泻儿童粪便标本,同时收集病例临床特征信息,标本冷增菌后利用耶尔森菌选择培养平板分离小肠结肠炎耶尔森菌,通过生物分型、血清学分型及毒力基因检测分析菌株病原学特征。结果共采集397份腹泻粪便标本,在3份标本中检出7株耶尔森菌,耶尔森菌感染率为0.76%(3/397)。3份耶尔森菌阳性标本中,第212号标本检出2株弗氏或中间型耶尔森菌,第24号和第226号标本中检出5株的耶尔森菌均为小肠结肠炎耶尔森菌,其中第24号标本检出2株小肠结肠炎耶尔森菌,为生物1A型,毒力基因检测为:ail-、ystA-、ystB+、yadA-、virF-,为非致病性小肠结肠炎耶尔森菌;第226号标本检出3株小肠结肠炎耶尔森菌,为生物3型、血清O∶3型(rfbc+),毒力基因检测为:ail+、ystA+、ystB-、yadA+、virF+,为致病性小肠结肠炎耶尔森菌。小肠结肠炎耶尔森菌感染率为0.50%(2/397),致病性小肠结肠炎耶尔森菌检自11月龄腹泻儿童粪便标本。结论云南省西部地区≤5岁儿童存在致病性小肠结肠炎耶尔森菌散发的感染状况,需加强小肠结肠炎耶尔森菌病的监测。Objective To investigate the prevalence and pathogenic characteristics of Yersinia enterocolitica infection in children with diarrhea under 5 years of age in western Yunnan,and to provide a basis for the prevention and treatment of infectious diarrhea in children.Methods Feces were collected from under five-year-old children with diarrhea in the First Affiliated Hospital of Dali University from 2020 to 2021.Clinical information of the cases was also collected.Yersinia enterocolitica was isolated from the samples after cold enrichment on selective culture plates,and the pathogenic characteristics of Yersinia enterocolitica were analyzed by biological type and serotype and virulence gene detection.ResultsA total of 397feces were collected.Seven strains of Yersinia enterocolitica were isolated in three samples,and the prevalence of Yersinia enterocolitica infection was 0.76%(3/397).Among the three positive samples,two Yersinia frederiksenii or Yersinia intermedia were isolated in specimen No.212,and five Yersinia enterocolitica were detected in specimens No.24 and 226.Two Yersinia enterocolitica isolated from one sample were biological type 1A,and the virulence gene test results were ail-/ystA-/ystB+/yadA-/virF-,which were non-pathogenic Yersinia enterocolitica.Three Yersinia enterocolitica isolated from the other sample were biological type 3,serotype O∶3(rfbc+),and virulence gene detection results were ail+/ystA+/ystB-/yadA+/virF+,which were pathogenic Yersinia enterocolitica.While pathogenic Yersinia enterocolitica was detected from feces of children with diarrhea at 11 months of age with a infection rate of 0.50%(2/397).Conclusion Sporadic infection of pathogenic Yersinia enterocolitica was found in under five-year-old children in western Yunnan Province.It is necessary to strengthen the monitoring and research of Yersinia enterocolitica.

关 键 词：儿童腹泻 小肠结肠炎耶尔森菌 致病性 调查 

分 类 号：R378.61[医药卫生—病原生物学]