肠道菌群与孤独症谱系障碍儿童功能性便秘的关系  

Correlation between gut microbiota and functional constipation in children with autism spectrum disorder

作　　者：李正 杨桂存 蒋静[1] 肖贵元 王念蓉[1] 赵妍[1] 何春燕[1] LI Zheng;YANG Gui-cun;JIANG Jing;XIAO Gui-yuan;WANG Nian-rong;ZHAO Yan;HE Chun-yan(Department of Pediatrics,Chongqing Health Center for Women and Children(Women and Children's Hospital of Chongqing Medical University Chongqing),Chongqing 400021,China)

机构地区：[1]重庆市妇幼保健院/重庆医科大学附属妇女儿童医院儿科,重庆400021

出　　处：《中国微生态学杂志》2023年第1期63-68,72,共7页Chinese Journal of Microecology

基　　金：重庆市科卫联合医学科研项目(2021MSXM176);国家妇幼中心母婴健康项目(2019FYH015)。

摘　　要：目的探讨孤独症谱系障碍(autism spectrum disorder,ASD)儿童肠道菌群组成与功能性便秘之间的关系,为该类患者的治疗提供参考。方法选择101例2~7岁ASD儿童和82例年龄、性别相匹配健康儿童为研究对象,采用罗马Ⅳ标准评估便秘,将所有儿童分为ASD便秘组(ASD constipated,AD-C)、ASD非便秘组(ASD non-constipated,AD-NC)、正常便秘组(neurotypical constipated,NT-C)、正常非便秘组(neurotypical non-constipated,NT-NC)。对所有粪便样本中细菌16S rRNA基因V3-V4高变区进行测序,检测肠道菌群。结果与NT组相比,AD组儿童肠道菌群α多样性指数(Chao1)显著升高(t=2.258,P=0.006),组间β多样性差异有统计学意义(R=0.210,P<0.001)。AD组儿童肠道优势菌群为双歧杆菌属、柯林斯菌属、脱硫弧菌属等。与NT-NC组相比,AD-NC组患儿肠道菌群Chao1指数显著升高(t=2.170,P=0.021),组间β多样性差异有统计学意义(R=0.123,P=0.002)。AD-NC组优势菌群同样包含脱硫弧菌。AD-C组和AD-NC组儿童肠道菌群β多样性差异有统计学意义(R=0.221,P<0.001)。AD-C组儿童肠道优势菌群为罗氏菌属、假丁基弧菌、Deinococcus等。结论ASD儿童较健康儿童肠道菌群存在差异,便秘可能增加孤独症患者肠道菌群的异质性特征,脱硫弧菌可能与ASD的发病机制相关。Objective To explore the relationship between gut microbiota and functional constipation in children with autism spectrum disorder(ASD)and provide a reference for the treatment.Methods This study enrolled 101 children with ASD aged 2-7 years and 82 sex-and age-matched neurotypical children in our hospital.The children were divided into ASD constipation group(AD-C),ASD non-constipation group(AD-NC),neurotypical constipation group(NT-C),and neurotypical non-constipation group(NT-NC).The V3-V4 hypervariable regions of bacterial 16S rRNA gene in fecal samples were sequenced.Results Compared with the NT group,the AD group had significant increase in intestinal flora Chao1 index(t=2.258,P=0.006).There was a significant difference in the structure of gut microbiota between AD group and NT group(R=0.210,P<0.001).Compared with NT group,the dominant bacteria were Bifidobacterium,Collinsella and Desulfovibrio in the AD group.Compared with the NT-NC group,the AD-NC group had significant increase in intestinal flora Chao1 index(t=2.170,P=0.021).There was a significant structural difference in the composition of gut microbiota between the AD-NC and NT-NC group(R=0.123,P=0.002).The dominant bacteria were Cardiobacterium,Bacteroides,Sutterella and Desulfovibrio in the AD-NC group.Compared with AD-NCgroup,the dominant bacteria were Roseburia,Pseudobutyrivibrio and Deinococcus in the AD-C group.Conclusion Functional constipation may enhance the heterogenous characteristics of gut microbiota in ASD.The genus Desulfovibrio may be more directly related to ASD pathogenesis.

关 键 词：孤独症谱系障碍 肠道菌群 功能性便秘 儿童 

分 类 号：R749.94[医药卫生—神经病学与精神病学]