卵巢癌组织中PCDH8、TSLC1基因启动子甲基化状态及其与患者预后的关系  

Methylation Status of PCDH8 and TSLC1 Gene Promoters in Ovarian Cancer Tissue and Relationship with Prognosis

作　　者：王蕊 刘阳[1] 邓云静 WANG Rui;LIU Yang;DNEG Yun-jing(Department of Obstetrics and Gynecology,Yulin First Hospital,Yulin Shaanxi 719000)

机构地区：[1]榆林市第一医院妇产科,陕西榆林719000

出　　处：《医学临床研究》2023年第2期209-212,共4页Journal of Clinical Research

摘　　要：【目的】探讨卵巢癌组织中原钙黏蛋白8(PCDH8)、肺癌抑癌基因1(TSLC1)基因启动子甲基化状态及其与患者预后的关系。【方法】回顾性分析2017年5月至2018年5月在本院行手术治疗的104例卵巢癌患者的临床资料,对所有患者术后均随访36个月,将随访期间出现病灶转移及复发、死亡的患者纳入预后不良组(n=60),余下的纳入预后良好组(n=44)。甲基化特异性聚合酶链反应(MSP)检测所有患者卵巢癌组织中PCDH8、TSLC1基因启动子甲基化状态,单因素分析和多因素分析卵巢癌患者预后不良的影响因素。【结果】预后不良组患者卵巢癌组织中PCDH8基因启动子、TSLC1基因甲基化率分别为80.00%(48/60)、58.33%(35/60),显著高于预后良好组患者的36.36%(16/44)、18.18%(8/44),差异有统计学意义(χ^(2)=20.422、16.875,P<0.05)。预后不良组FIGO分期、淋巴结有无转移、分化程度、PCDH8基因启动子甲基化状态、TSLC1基因启动子甲基化状态与预后良好组比较,差异有统计学意义(P<0.05)。FIGO分期Ⅲ~Ⅳ期、淋巴结有转移、分化程度G3、PCDH8基因启动子甲基化状态阳性、TSLC1基因启动子甲基化状态阳性均是卵巢癌患者预后不良的危险因素(P<0.05)。【结论】预后不良的卵巢癌患者癌组织中PCDH8、TSLC1基因启动子甲基化阳性率明显高于预后良好患者,PCDH8、TSLC1基因启动子甲基化状态与其预后显著相关,二者均可评估卵巢癌患者预后。【Objective】To investigate the methylation status of the promoter of PCDH8 and TSLC1 genes in ovarian cancer tissue and relationship with prognosis.【Methods】The clinical data of 104 patients with ovarian cancer who underwent surgical treatment in our hospital from May 2017 to May 2018 were retrospectively analyzed.All patients were followed up for 36 months after surgery.The patients with metastasis,recurrence and death during the follow-up period were set as the poor prognosis group(n=60),and the rest were the good prognosis group(n=44).Methylation-specific polymerase chain reaction(MSP)was used to detect the methylation status of PCDH8 and TSLC1 gene promoters in ovarian cancer tissues of all patients.Univariate analysis and multivariate analysis were used to analyze the factors affecting the poor prognosis of ovarian cancer patients.【Results】The methylation rates of PCDH8 gene promoter and TSLC1 gene promoter in ovarian cancer tissue of patients with poor prognosis were 80.00%(48/60)and 58.33%(35/60),respectively,which were significantly higher than those of patients with good prognosis 36.36%(16/44)and 18.18%(8/44),respectively.The difference was statistically significant(χ^(2)=20.422、16.875,P<0.05).There were statistically significant differences in FIGO stage,lymph node metastasis,degree of differentiation,PCDH8 gene promoter methylation status,and TSLC1 gene promoter methylation status between the poor prognosis group and the good prognosis group(P<0.05).FIGO stageⅢ~Ⅳ,lymph node metastasis,degree of differentiation G3,positive PCDH8 and TSLC1 gene promoter methylation status were all risk factors for the poor prognosis of ovarian cancer patients(P<0.05).【Conclusion】The positive rates of both PCDH8 and TSLC1 gene promoter methylation in the cancer tissue of patients with poor prognosis are significantly higher than those of patients with good prognosis.The methylation status of both PCDH8 and TSLC1 gene promoters is significantly correlated with the prognosis and can be used to evaluat

关 键 词：卵巢肿瘤/病理学 卵巢肿瘤/遗传学 细胞粘附分子1 启动区 遗传学 DNA甲基化 

分 类 号：R737.31[医药卫生—肿瘤]