糖基化-酰化菜籽蛋白抗菌水凝胶的制备与表征  

Preparation and Characterization of Glycosylated-Acylated Rapeseed Protein Antibacterial Hydrogel

作　　者：王志高 陶璇 张倩玉 何荣 鞠兴荣 Wang Zhigao;Tao Xuan;Zhang Qianyu;He Rong;Ju Xingrong(College of Food Science and Engineering,Collaborative Innovation Center for Modern Grain Circulation and Safety,Key Laboratory of Grains and Oils Quality Control and Processing,Nanjing University of Finance and Economics,Nanjing 210023)

机构地区：[1]南京财经大学食品科学与工程学院,江苏省现代粮食流通与安全协同创新中心,江苏高校粮油质量安全控制及深加工重点实验室,南京210023

出　　处：《中国粮油学报》2022年第12期142-149,共8页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金项目(32101872);江苏省自然科学基金项目(BK20200837);江苏省农业自主创新项目[CX(21)3041]。

摘　　要：菜籽蛋白在食品的加工、储藏及配制等过程中表现出一定的成膜性、持水性、溶解性、和凝胶性等功能特性,可被用于蛋白基水凝胶的制备。然而蛋白基水凝胶的机械性和抑菌效果差,不利于伤口愈合。因此,本研究采用聚六亚甲基双胍盐酸盐(PHMB)来改善菜籽蛋白水凝胶的抗菌性能。首先通过糖基化协同酰化修饰菜籽蛋白,再经过热诱导法负载PHMB来制备抗菌水凝胶。结果显示制备的改性菜籽蛋白水凝胶可以显著抑制金黄色葡萄球菌和铜绿假单细胞菌的生长,抑菌圈直径分别达到8 mm和6 mm。同时加载PHMB未对菜籽蛋白复合水凝胶的透气性和溶胀性产生显著影响。此外,傅里叶红外光谱和X射线衍射图表明,加载PHMB前后菜籽蛋白水凝胶的吸水性能、热稳定性几乎没有发生变化。Rapeseed protein exhibits certain functional properties, such as film-forming, water-holding, solubility, and gelling properties during food processing, storage, and preparation, and can be used in the preparation of protein-based hydrogels. However, protein-based hydrogels have poor mechanical and bacteriostatic effects, etc, not conducive to wound healing. Therefore, in this study, polyhexamethylene biguanide hydrochloride(PHMB) was employed to improve the antibacterial properties of rapeseed protein hydrogels. Firstly, rapeseed protein was modified by glycosylation and synergistic acylation, and then, PHMB was loaded by thermal induction method to prepare antibacterial hydrogels. The results indicated that the prepared modified rapeseed protein hydrogel could significantly inhibit the growth of Staphylococcus aureus and Pseudomonas aeruginosa, and the diameter of the inhibition zone reached 8 mm and 6 mm, respectively. Meanwhile, loading of PHMB did not significantly affect the air permeability and swelling properties of rapeseed protein composite hydrogels. In addition, Fourier transform infrared spectroscopy and X-ray diffraction showed that the water absorption properties and thermal stability of rapeseed protein hydrogels did not change before and after loading with PHMB.

关 键 词：菜籽蛋白 复合水凝胶 抗菌 

分 类 号：TS210.2[轻工技术与工程—粮食、油脂及植物蛋白工程]