含BAG-nMgO的新型牙科复合树脂的抗菌及再矿化性能研究  

Study on antibacterial and remineralization properties of a novel dental resin composite containing BAG-nMgO

作　　者：田静[1] 武忠圆 王源[1] 郭迪 周子璇 付静[1] TIAN Jing;WU Zhong-yuan;WANGYuan;GUO Di;ZHOU Zi-xuan;FU Jing(Department of Prosthodontics,The Affiliated Hospital of Qingdao University,Qiangdao 266000,China;School of Stomatology of Qiangdao University,Qiangdao 266003,China;Department of Prosthodontics,West China School of Stomatology,Sichuan University,Chengdu 610000,China)

机构地区：[1]青岛大学附属医院口腔修复科、青岛大学口腔医学院,青岛266000 [2]四川大学华西口腔医院口腔修复科,成都610000

出　　处：《口腔颌面修复学杂志》2023年第2期99-106,共8页Chinese Journal of Prosthodontics

基　　金：国家自然科学基金(项目编号:51703106);山东省自然科学基金(项目编号:ZR2016EMQ05、ZR2022ME021);齐鲁卫生与健康杰出青年人才计划项目。

摘　　要：目的:联合应用生物活性玻璃(BAG)和纳米氧化镁(nMgO)改性牙科光固化复合树脂,以获得兼备优良的抗菌性及再矿化性能的新型牙科复合树脂。方法:将不同含量的BAG(2.5 wt%、7.5 wt%、12.5 wt%)和2.5 wt%nMgO引入牙科光固化复合树脂中,制备成含BAG-nMgO的实验树脂,并以卡瑞斯玛复合树脂作为对照。通过菌落计数、代谢活性分析(MTT)和活/死细菌染色,评价实验树脂的抗变形链球菌(S.mutans)能力;将脱矿人牙牙本质片及实验树脂在模拟体液(SBF)中浸泡21 d后,通过扫描电子显微镜(SEM)和X射线能谱仪(EDS)观察分析脱矿牙本质表面形貌和元素组成,评估实验树脂再矿化特性;通过万能试验机、维氏硬度仪等测试树脂的固化深度、压缩强度及维氏硬度。结果:实验组树脂的抗S.mutans活性显著高于卡瑞斯玛对照组,且与BAG的含量相关;当BAG添加量为7.5 wt%时,脱矿牙本质开始矿化;BAG添加比例为12.5 wt%时,可见厚的矿化层,牙本质小管几乎被完全封闭;实验组的固化深度均显著高于卡瑞斯玛,维氏硬度优于或与其相近,随着BAG比例的增加,各实验组的固化深度、压缩强度间无显著性差异。结论:BAG和nMgO的联合应用提高了实验树脂的抗菌性,并赋予其再矿化性能。新型复合树脂预期可减少细菌入侵和促进早期龋损的再矿化。本研究为新型多功能复合树脂的研究开发提供了理论依据和研究基础。Objective:To obtain a novel dental resin composite with better antibacterial and mineralization properties,bioactive glass(BAG)and nano-magnesium oxide(nMgO)were combined to introduce into the dental resin composite.Methods:Different content of BAG(2.5 wt%,7.5 wt%,12.5 wt%)and 2.5 wt%n Mg O were added into the dental light-curing resin composite as the experimental groups,and commercial Karisma was used as control.The ability of resistance to Streptococcus mutans(S.mutans)was evaluated by colony counting,metabolic activity analysis(MTT)and live/dead bacterial staining tests.The demineralized human dentin slices and experimental resins were immersed in simulated body fluid(SBF)for 21 d.The surface morphology and elemental composition of demineralized dentin tablets were observed and analyzed by scanning electron microscopy(SEM)and X-ray energy spectrometry(EDS),then the remineralization characteristics of the experimental resin composites were evaluated.The curing depth,compression strength and Vickers hardness of experimental resin composite were tested by a universal testing machine and Vickers hardness tester.Results:The antibacterial activity of the experimental groups were significantly higher than that of the control group,and it is correlated with the content of BAG.When BAG was 7.5 wt%,the demineralized dentin started to mineralize.Besides,when the ratio was increased to12.5 wt%,a thick mineralized layer was visible on the surface of demineralized dentin flakes,and the dentin tubules were almost completely closed.The curing depths of the experimental group were all significantly higher than that of the control group,and the Vickers hardness was similar to or better than it,with the increase of BAG.There was no significant difference between the curing depth and compression strength of the experimental groups.Conclusion:The combination of BAG and nMgO improved the antibacterial activity and remineralization properties of the novel resin composite,which is expected to reduce bacterial invasion and promote re

关 键 词：BAG nMgO 抗菌性 再矿化 光固化复合树脂 

分 类 号：R783.1[医药卫生—口腔医学]