黑布药膏的化学成分表征研究  

Characterization of chemical constituents of Heibu Yaogao(ointment)

作　　者：成小红 刘惠君 蒋丽 刘丽芳 CHENG Xiaohong;LIU Huijun;JIANG Li;LIU Lifang(Hospital for Skin Diseases,Chinese Academy of Medical Sciences,Nanjing 210042;Department of Pharmacognosy,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]中国医学科学院皮肤病医院,南京210042 [2]中国药科大学中药学院生药学系,南京211198

出　　处：《中国药科大学学报》2023年第1期77-88,共12页Journal of China Pharmaceutical University

摘　　要：黑布药膏系常用的院内制剂,由五倍子、蜈蚣等制成,临床疗效确切,但其药效物质基础尚未全面阐明。本研究采用超高效液相色谱-高分辨飞行时间质谱联用技术(UPLC-Q-TOF-MS/MS)对黑布药膏及其生药混合物的化学成分进行定性分析,并探讨了制剂前后成分的差异。在电喷雾(ESI)正、负离子模式下,根据精确相对分子质量、特征碎片离子、色谱保留时间等初步鉴定出73个化合物,其中生药混合物、黑布药膏分别鉴定了57、65个化合物,包括鞣质类、酚酸类、核苷类、氨基酸类等,在制备过程中,化学结构发生变化的成分主要集中在鞣质类。结果表明,UPLC-Q-TOF-MS/MS技术可以灵敏、高效地表征黑布药膏制剂中所含的化学成分及其在制剂过程中的结构变化,为充分阐明黑布药膏药效物质基础和制剂工艺的合理性及后续制剂质量标准提升研究提供重要依据。Heibu Yaogao(ointment),which made of Galla chinensis,Scolopendra,etc.,is a commonly used hospital preparation with definite clinical efficacy,yet its material basis has not been fully clarified.By ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS),the chemical constituents of Heibu Yaogao(ointment)and its crude drug mixture were qualitatively analyzed,and the differences of components before and after preparation were discussed.Scanned by electrospray ionization(ESI)source in positive and negative ion modes,a total of 73 compounds were identified via precise molecular weight,characteristic fragment ions and chromatographic retention time.57 and 65 compounds were detected in crude drug mixture and Heibu Yaogao(ointment)respectively,including tannins,phenolic acids,nucleosides,amino acids,etc.In the process of preparation,the chemical structure changes are mainly concentrated in tannins.The change of components of Heibu Yaogao(ointment)before and after processing were proved by UPLC-QTOF-MS/MS,which provides important reference for elucidating the material basis of Heibu Yaogao(ointment),illustrating the rationality of preparation technology and further promoting the quality control of the preparation.

关 键 词：黑布药膏 五倍子 蜈蚣 UPLC-Q-TOF-MS/MS 化学成分 

分 类 号：R914[医药卫生—药物化学]