壁虎抗肿瘤活性成分的提取分离及抗血管生成作用研究  

Study on the extracted of anti-tumor active components from gecko and the mechanism of antiangiogenesis

作　　者：韩明[1] 连波[2] 程鑫[3] HAN Ming;LIAN Bo;CHENG Xin(Weifang Medical University,School of Basic Medical Sciences,Division of Preclinical Medicine Theories,Weifang 261053,China;Weifang Medical University,School of Bioscience and Technology,Experimental Center of Life Science and Technology;Weifang Medical University,School of Medical Imaging,Imaging Medical Diagnosis Teaching and Research Office)

机构地区：[1]潍坊医学院基础医学院基础医学概论教研室,山东潍坊261053 [2]潍坊医学院生命科学与技术学院生命科学与技术实验教学中心 [3]潍坊医学院医学影像学院影像医学诊断教研室

出　　处：《潍坊医学院学报》2022年第5期321-323,共3页Acta Academiae Medicinae Weifang

基　　金：山东省中医药科技发展计划项目(项目编号:2019-0415);山东省中医药科技项目(项目编号:2021M131)。

摘　　要：目的提取壁虎中抗肿瘤的有效成分,并对其抗肿瘤机制进行初步探讨。方法新鲜壁虎经过酒精浸泡,粉碎、过滤、旋蒸、抽滤,有机溶剂萃取。在细胞实验的指导下,利用高效液相色谱法分离后,最终提取分离到具有抗肿瘤作用的活性成分。通过大鼠角膜袋实验对其抗肿瘤的作用机制进行初步研究。结果实验提取的壁虎抗肿瘤活性成分有较强的抗肿瘤作用,并可以抑制由VEGF诱导的角膜新生血管的生成。结论该方法可以提取到壁虎中的抗肿瘤活性成分。该抗肿瘤成分能够通过抑制新生血管的生成达到抗肿瘤的作用。Objective To extract effective anti-tumor components from gecko,and explore its anti-tumor mechanism preliminarily.Methods Fresh gecko was soaked in alcohol,crushed,filtered,steamed,filtered,and extracted with organic solvents.Under the guidance of cell experiment,the active components with anti-tumor effect were finally extracted and separated by high performance liquid chromatography.The mechanism of its anti-tumor effect was preliminarily studied by rat corneal pouch experiment.Results The results showed that the anti-tumor active ingredients extracted from gecko could inhibit the corneal neovascularization induced by VEGF.Conclusion This method can extract the anti-tumor active components from gecko.The anti-tumor component has the effect of anti-tumor by inhibiting the production of new blood vessels.

关 键 词：壁虎 肿瘤 角膜袋 血管生成 

分 类 号：R730.23[医药卫生—肿瘤]