ZNF804A基因多态性与维吾尔族首发精神分裂症患者精神症状严重程度的关联研究  

Association between ZNF804A polymorphism and severity of psychiatric symptom of Uighur first-episode schizophrenic patients

作　　者：孙乐乐 史新玉 徐斌[1] 胡红星[1] 母代斌[1] 傅松年[1] 师咏勇[2] 伊琦忠[1] Sun Lele;Shi Xinyu;Xu Bin;Hu Hongxing;Mu Daibin;Fu Songnian;Shi Yongyong;Yi Qizhong(Psychological Medicine Center,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;Bio-X Research Institute,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]新疆医科大学第一附属医院心理医学中心,乌鲁木齐830054 [2]上海交通大学Bio-X研究院,200240

出　　处：《神经疾病与精神卫生》2023年第1期11-17,共7页Journal of Neuroscience and Mental Health

基　　金：国家自然科学基金(81660233,81360209,81960258);新疆医科大学创新创业项目(CXCY2017031)。

摘　　要：目的了解锌指蛋白804A基因(ZNF804A)基因rs13009002、rs72893068、rs61739291 3个位点的基因多态性,并分析与维吾尔族首发精神分裂症患者精神症状严重程度的关系。方法于2015年7月至2018年6月,选取新疆维吾尔自治区喀什、和田精神卫生机构及新疆医科大学第一附属医院精神科收治的937例维吾尔族首发精神分裂症患者以及937名健康体检者为病例组和对照组。采用PANSS评估病例组精神症状的严重程度。采用高通量基因测序技术对两组ZNF804A基因rs13009002、rs72893068、rs61739291 3个位点进行测序分析,并对关联位点进行性别比较,比较基因多态性与精神症状严重程度。结果两组受试者rs72893068和rs61739291位点的基因型和基因频率比较,差异无统计学意义(P>0.05)。两组受试者rs13009002位点G基因频率为92.2%(1 728/1 874),高于对照组的90.2%(1 690/1 874),差异有统计学意义(χ^(2)=4.798,P<0.05)。两组受试者rs13009002位点显性[OR(95%CI)=1.30(1.02~1.66)]、超显性[OR(95%CI)=1.28(1.00~1.63)]和加性[OR(95%CI)=1.31(1.03~1.65)]遗传模式比较,差异有统计学意义(P<0.05)。在男性受试者中,病例组rs13009002位点显性GG基因型、超显性GG/TT基因型多于对照组,差异有统计学意义(χ^(2)=11.944、11.645;P<0.01)。在男性受试者中,病例组G基因频率高于对照组,差异有统计学意义(χ^(2)=11.020,P<0.01)。男性患者阴性症状得分高于女性,一般精神病理、抑郁和激活性得分低于女性,差异有统计学意义(t=2.003、2.023、3.296、2.736;P<0.05);青年、中年、老年患者的阳性症状和思维障碍得分比较,差异有统计学意义(F=4.136、5.238;P<0.05)。病例组rs13009002显性GG基因型男性患者的阳性症状、思维障碍得分高于GT/TT基因型,差异有统计学意义(t=2.970、2.260;P<0.05);超显性GG/TT基因型男性患者的阳性症状、思维障碍和攻击性得分高于GT基因型患者,差异有统计学意义(t=2.776、2.244Objective To explore the genetic polymorphisms of 3 sites,including rs13009002,rs72893068 and rs61739291,of zinc finger protein 804A gene (ZNF804A),and to analyze the correlation between ZNF804A polymorphism and severity of symptoms in Uighur first-episode schizophrenic patients.Methods From July 2015 to June 2018,a total of 937 Uighur first-episode schizophrenic patients and 937healthy physical examinees admitted in the Kashgar and Hotan Mental Health Institutions of Xinjiang Uygur Autonomous Region and the Department of Psychiatry of the First Affiliated Hospital of Xinjiang Medical University were selected as the case group and the control group.The severity of psychotic symptoms was assessed using the Positive and Negative Syndrome Scale (PANSS).The 3 loci (rs13009002,rs72893068and rs61739291) of ZNF804A of the 2 groups were sequenced using high-throughput gene sequencing.The association loci were compared by gender.The gene polymorphisms and severity of psychiatric symptoms were compared.Results There were no significant differences between the case and control groups in genotyping of rs72893068 and rs61739291,or allele frequency (P>0.05).The G allele frequency of rs13009002 of the case group was 92.2%(1 728/1 874),higher than that of the control group 90.2%(1 690/1 874),and the difference was statistically significant(χ^(2)=4.026,P<0.05).The differences in dominant[OR (95%CI)=1.30 (1.02-1.66)],over-dominant[OR (95%CI)=1.28 (1.00-1.63)],and additive[OR (95%CI)=1.31 (1.03-1.65)]genetic patterns of rs13009002 between the 2 groups were statistically significant (P<0.05).Among the male subjects,the dominant GG genotype and over-dominant GG/TT genotype at rs13009002 locus in the case group were more than those in the control group,and the differences were statistically significant(χ^(2)=11.944,11.645;P<0.01).Among the male subjects,the frequency of G gene in the case group was higher than that in the control group,and the difference was statistically significant(χ^(2)=11.020,P<0.01).The score of negative sympt

关 键 词：精神分裂症 锌指蛋白804A基因 基因多态性 维吾尔族 

分 类 号：R749.3[医药卫生—神经病学与精神病学]