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作 者:高莎莎 宋苗苗 冯洁 王会生 王勇 GAO Shasha;SONG Miaomiao;FENG Jie;WANG Huisheng;WANG Yong(Department of Physiology and Pathophysiology,School of Basic Medical Sciences,Institute of Neuroscience,Xi’an Jiaotong University Health Science Center,Xi’an 710061,China)
机构地区:[1]西安交通大学医学部基础医学院生理学与病理生理学系,西安710061
出 处:《山西医科大学学报》2023年第1期69-74,共6页Journal of Shanxi Medical University
基 金:国家自然科学基金面上项目(82071526)。
摘 要:目的探讨丘脑室旁核(PVT)内多巴胺D2受体阳性神经元在大鼠焦虑样行为调节中的作用。方法采用腹腔注射脂多糖(0.2 mg/kg LPS)的方法构建炎性相关焦虑行为大鼠模型。对照组(control)接受等量盐水注射。通过比较LPS组(n=6)和control组(n=6)动物在旷场中央区和高架十字开放臂停留时间的差异,评价LPS组动物焦虑水平的变化。将LPS注射建立的焦虑模型大鼠,分为普拉克索组(n=6)和生理盐水组(n=6),观察PVT内微量注射D2受体激动剂普拉克索(6μg/μl)对动物焦虑样行为的影响。将D2特异性环化重组酶大鼠分为光敏感通道蛋白组(ChR2,n=6)和红色荧光蛋白组(mCherry,n=6)。采用470 nm光选择性刺激两组大鼠的PVT,观察两组动物焦虑水平的差异。结果与control组相比,LPS组大鼠出现焦虑样行为,在中央区停留时间缩短(P=0.002),开放臂停留时间减少(P=0.004)。与生理盐水组比较,普拉克索组大鼠的焦虑样行为减轻,在中央区停留时间增长(P=0.008),开放臂停留时间增加(P=0.041)。与mCherry组比较,ChR2组大鼠出现焦虑样行为,在中央区停留时间缩短(P=0.013),开放臂停留时间减少(P=0.018)。结论PVT内多巴胺D2受体阳性神经元的过度激活促进大鼠焦虑样行为的产生。Objective To explore the role of the paraventricular thalamic nucleus(PVT)dopamine D2 receptor-positive neurons in the regulation of anxiety-like behaviors in rats.Methods A rat model of inflammation-related anxiety was established by intraperitoneal injection of lipopolysaccharide(0.2 mg/kg LPS).The rats in control group received the same volume of saline injection.The time in the central area in the open field test and the time on the open arm of the elevated plus maze were compared between LPS group(n=6)and control group(n=6)to evaluate the anxiety level.The LPS-induced anxiety model rats were divided into pramipexole group(n=6)and normal saline group(n=6),and the effects of intra-PVT microinjection of D2 receptor agonist pramipexole on anxiety-like behavior of rats were observed.The D2-Cre rats were divided into ChR2 group(n=6)and mCherry group(n=6).The 470 nm light was used to selectively activate PVT of rats in ChR2 group and mCherry group,and the difference of anxiety level was observed between two groups.Results Compared with control group,the obvious anxiety-like behaviors of rats were found in LPS group,and the time in the central area was shortened(P=0.002),and the time on the open arm was reduced(P=0.004).Compared with normal saline group,the anxiety-like behaviors were inhibited in rats in pramipexole group,and the time in center was increased(P=0.008),and the time on the open arm was increased(P=0.041).Compared with mCherry group,the anxiety-like behaviors of rats in ChR2 group was increased,and the time in center was shortened(P=0.013),and the time on the open arm was reduced(P=0.018).Conclusion Overactivation of dopamine D2 receptor-positive neurons in PVT can promote the anxiety-like behaviors in rats.
关 键 词:焦虑样行为 多巴胺D2受体 丘脑室旁核 神经元 脂多糖 多巴胺受体激动剂 大鼠
分 类 号:R749.72[医药卫生—神经病学与精神病学]
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