水稻种质资源稻瘟病抗性全基因组关联分析  

Identification of rice blast resistance in xian and geng germplasms by genomewide association study

作　　者：周海平 张帆[2] 陈凯 申聪聪 朱双兵 邱先进[4] 徐建龙[2,3,5] ZHOU Hai-Ping;ZHANG Fan;CHEN Kai;SHEN Cong-Cong;ZHU Shuang-Bing;QIU Xian-Jin;XU Jian-Long(Wenzhou Academy of Agricultural Sciences/South-Zhejiang Crop Breeding Key Laboratory,Wenzhou 325000,Zhejiang;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,Guangdong,China;College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China;Hainan Yazhou Bay Seed Laboratory,Sanya 572024,Hainan,China)

机构地区：[1]温州市农业科学研究院/浙南作物育种重点实验室,浙江温州325000 [2]中国农业科学院作物科学研究所,北京100081 [3]岭南现代农业科学与技术广东省实验室深圳分中心/中国农业科学院农业基因组研究所,广东深圳518210 [4]长江大学农学院,湖北荆州434025 [5]海南省崖州湾种子实验室,海南三亚572024

出　　处：《作物学报》2023年第5期1170-1183,共14页Acta Agronomica Sinica

基　　金：海南崖州湾种子实验室揭榜挂帅项目(B21HJ0216);温州市农业新品种选育协作组专项(2019ZX006)资助。

摘　　要：稻瘟病是一种对全球水稻生产威胁极大的真菌性病害,鉴定抗稻瘟病基因并将其导入到现有感病品种改良品种的抗性是控制这种病害的有效途径。本研究利用5个稻瘟病菌株鉴定了212份籼稻和235份粳稻种质资源的苗瘟抗性,分别筛选到8个和12个抗全部5个菌株的籼稻和粳稻种质材料。采用全基因组关联分析在籼粳混合群体、籼稻和粳稻种质资源中共定位到43个影响水稻苗瘟抗性的QTL,抗GD00-193、GD08-T19、GD17-CQ16、HB1708和HLJ13-856菌株的QTL分别为9、4、14、14和2个。其中, 12个抗病QTL仅在籼稻亚群中检测到, 7个仅粳稻亚群中检测到,1个为籼粳2个亚群共同检测到,说明籼稻抗稻瘟病总体好于粳稻,而且稻瘟病抗性存在明显的籼粳分化。同时影响水稻对2个及2个以上菌株的抗性或在2个及2个以上群体中同时被定位到的QTL共计11个,利用候选区间关联分析和单倍型分析鉴定到23个抗病候选基因,不同抗病候选基因在籼、粳群体中的分布频率不同。研究结果为水稻品种稻瘟病抗性分子改良提供种质资源和有利基因信息及不同抗病基因的育种利用策略。Rice blast is one of the major fungal diseases that threaten rice production worldwide. To improve rice blast resistance,identifying blast resistant genes and introgressing them into elite rice varieties is an effective way. In this study, a panel of 212xian accessions and 235 geng accessions collected worldwide were evaluated for resistance against five blast isolates at seedling stage. All of them showed large variations in resistance against five isolates, and 8 xian and 12 geng accessions were detected to present resistance to all five blast isolates. Using genome-wide association strategy, a total of 43 QTLs were identified for resistance to five isolates in mix population(xian subpopulation and geng subpopulation), including 9, 4, 14, 14, and 2 QTLs for GD00-193, GD08-T19, GD17-CQ16, HB1708, and HLJ13-856, respectively. Among them, 12 resistant QTLs were detected only in xian rice sub-population, 7 only detected in geng rice sub-population, and 1 simultaneously detected in both sub-populations,indicating that blast resistance was generally better in xian than in geng rice, and there was obvious differentiation in blast resistance between xian and geng rice. A total of 11 QTLs affected resistance to two or more trains or were simultaneously identified in two or more populations, and 23 candidate genes were identified by candidate interval association analysis and haplotype analysis.Different resistance candidate genes had different frequencies in xian and geng populations. The results provide germplasm resources and favorable genes information for molecular improvement of blast resistance in rice varieties and the breeding and utilization strategies of different resistance genes.

关 键 词：稻瘟病抗性 种质资源 全基因组关联分析 数量性状位点 有利等位基因 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]