钛表面不同去污方式的体外效果比较  

Effects of different debridement methods on titanium surfaces in vitro

作　　者：葛晓彤 叶庆元 王津津 张曦戈 王垭铮 王晓雨 冀吉昀 王勤涛 GE Xiaotong;YE Qingyuan;WANG Jinjin;ZHANG Xige;WANG Yazheng;WANG Xiaoyu;JI Jiyun;WANG Qintao(State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture,Department of Periodontology,School of Stomatology,Air Force Medical University of PLA,Xi'an 710032,China;State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Key Laboratory of Stomatology,Digital Dentistry Center,School of Stomatology,Air Force Medical University of PLA,Xi'an 710032,China)

机构地区：[1]军事口腔医学国家重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔生物工程技术研究中心,空军军医大学口腔医院牙周病科,陕西西安710032 [2]军事口腔医学国家重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,空军军医大学口腔医院数字化口腔医学中心,陕西西安710032

出　　处：《口腔疾病防治》2023年第7期465-472,共8页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金面上项目(82170955);国家自然科学基金重点项目(81530051)。

摘　　要：目的探讨光动力疗法、喷砂、钛刮匙不同的去污方式对钛表面形貌及细菌黏附的影响,为种植体周围病的治疗提供参考。方法分别将牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)和具核梭杆菌(Fusobacterium nucleatum,Fn)接种于经抛光处理的钛试样表面,培养后使用不同去污方式对钛试样表面进行处理。根据不同去污方式将钛试样分为无处理对照组、光动力组、喷砂组、钛刮匙组。原子力显微镜观测钛表面粗糙度变化,扫描电镜、活/死菌染色实验观察钛表面剩余细菌情况;在去污后的钛试样表面重新接种Pg和Fn后观察细菌再黏附情况。结果原子力显微镜观测表面粗糙度结果显示钛刮匙组显著高于对照组、光动力组及喷砂组(P<0.05),对照组、光动力组及喷砂组组间差异均无统计学意义(P>0.05)。接触角测量结果显示各处理组表面接触角均小于对照组(P<0.05)。扫描电镜结果显示,钛试样表面去污后对照组表面剩余细菌数量较多,且细菌相对集中,光动力组、喷砂组、钛刮匙组表面细菌散落分布,数量较少,且光动力组表面大部分细菌菌体破裂。活/死菌染色实验结果显示光动力组表面死菌比例显著高于对照组、喷砂组及钛刮匙组(P<0.05),喷砂组和钛刮匙组表面剩余细菌以活菌为主;喷砂组表面剩余细菌黏附量与对照组及光动力组、钛刮匙组相比显著减少(P<0.05)。钛试样表面细菌再黏附的扫描电镜及活/死菌染色结果显示,钛刮匙组表面牙龈卟啉单胞菌有聚集现象,其表面细菌黏附量显著高于对照组、光动力组及喷砂组(P<0.05)。结论喷砂去污效果优于光动力疗法和钛刮匙,但并不能完全清除细菌污染;光动力疗法杀菌效果优于喷砂和钛刮匙;在临床实践中可以考虑喷砂和光动力疗法相结合的方法治疗种植体周围病。Objective To investigate the effect of different decontamination methods,including photodynamic therapy,sandblasting and titanium curette,on titanium surface morphology and bacterial adhesion for the treatment of periimplant disease.Methods Porphyromonas gingivalis(Pg)and Fusobacterium nucleatum(Fn)were inoculated on the surface of polished titanium specimens,and titanium specimen surfaces were treated with different decontamination methods after incubation.The titanium specimens were divided into a no-treatment control group,photodynamic group,sandblasting group and titanium curette group according to different decontamination methods.The changes in titanium surface roughness were observed by atomic force microscopy(AFM),and the remaining bacteria on the titanium surface were observed by scanning electron microscopy(SEM)and live/dead bacteria staining tests.After reinoculation of Pg and Fn,bacterial readhesion was observed on the surface of decontaminated titanium specimens.Results The AFM results showed that the surface roughness of the titanium curette group was significantly higher than that of the no-treatment control group,photodynamic group and sandblasting group(P<0.05),and there was no statistically significant difference between the no-treatment control group,photodynamic group and sandblasting group(P>0.05).The results of contact angle measurement showed that the surface contact angle of each treatment group was smaller than that of the no-treatment control group(P<0.05).The SEM results obtained after the titanium specimen surface was decontaminated showed that the number of bacteria on the no-treatment control group surface was higher and the bacteria were relatively concentrated.The bacteria on the surface of the photodynamic group,sandblasting group and titanium curette group were scattered and distributed in small numbers,and most bacteria on the surface of the photodynamic group were ruptured.The results of the live/dead bacteria staining experiment showed that the percentage of dead bacteria on t

关 键 词：种植体周围炎 牙龈卟啉单胞菌 具核梭杆菌 光动力疗法 喷砂 钛刮匙 种植体 粗糙度 菌斑 去污 细菌黏附 

分 类 号：R78[医药卫生—口腔医学]