SLA-Bio处理对纯钛表面MC3T3-E1细胞黏附行为的影响  

Effects of SLA-Bio treatment on adhesion behavior of MC3T3 E1 cells on pure titanium surface

作　　者：周冠军[1] 于晴 赵甜甜 王叶青 梅双 ZHOU Guanjun;YU Qing;ZHAO Tiantian(Department of Orthodontics,the Second Hospital of Hebei Medical University Hebei,Shijiazhuang 050017,China;不详)

机构地区：[1]河北医科大学第二医院口腔正畸科,石家庄市050017 [2]河北医科大学口腔医学院,口腔医院口腔颌面外科,河北省口腔医学重点实验室

出　　处：《河北医药》2023年第1期52-55,共4页Hebei Medical Journal

基　　金：河北医科大学自然科学领域青年科技人才支持计划项目(编号:CYQD202003)。

摘　　要：目的在传统喷砂酸蚀(SLA)处理基础上对纯钛表面进行喷砂、蚀刻和生物矿化(SLA-Bio)处理,观察其表面MC3T3-E1前成骨细胞的黏附铺展行为,以期为纯钛种植体表面改性提供更多研究方向。方法将纯钛片分为SLA组(TiO_(2)砂粒喷砂+HCI/H_(2)SO_(4)混合液酸蚀)和SLA-Bio组(SLA处理后再进行矿化处理14 d),通过SEM-EDS观察钛片表面形貌并分析其表面元素构成。于2组钛片表面接种MC3T3-E1细胞,分别于接种后1 h、6 h、24 h取出钛片,MTT法计算并比较2组钛片表面细胞黏附率,通过荧光染色观察、Image Pro Plus软件分析计算,比较不同表面MC3T3-E1细胞铺展行为及形态因子的差异。结果SLA组钛片表面呈蜂窝状、大小不一、深浅不等的不规则孔洞结构,SLA-Bio组钛片表面可见多孔微纳米球状羟磷灰石(hydroxyapatite,HA)沉积物,球体表面可见针状纳米微粒;MC3T3-E1细胞接种后1 h、6 h、24 h时,SLA-Bio组细胞黏附率均明显高于SLA组(P<0.05),SLA-Bio组细胞形态因子均明显低于SLA组(P<0.05);细胞荧光染色结果显示,与SLA组相比,SLA-Bio组在接种后各个时间段均呈现更好的黏附伸展形态。结论SLA-Bio处理可在纯钛表面形成多孔微纳米球状HA沉积物;SLA-Bio表面较SLA表面更有利于MC3T3-E1细胞的黏附和伸展,可能促进种植体骨结合的过程。Objective To observe adhesion and spreading behaviors of MC3T3 E1 cells on the pure titanium surface by sand blast and acid etching and biomineralization(SLA-Bio) treatment based on traditional sand blast and acid etching(SLA) treatment, aiming to provide more research orientation for modification of pure titanium surfaces.Methods Pure titanium sheets were divided into the SLA group(TiO_(2) sand blasting + HCI/H_(2)SO_(4) mixed solution etching) and SLA-Bio group(mineralization treatment for 14 days after SLA treatment),the surface morphology of titanium sheets was observed, and the surface element composition was analyzed by SEM-EDS. MC3T3-E1 cells were inoculated on the titanium sheet surfaces in 2 groups, and titanium sheets were taken out in 1h, 6h and 24h after inoculation. The adhesion rate of cells on titanium sheet surfaces in groups was calculated and compared by MTT method. Differences in the spreading behavior and morphological factors of MC3T3-E1 cells on different surfaces were compared by fluorescence staining and Image-Pro Plus software.Results The titanium sheet surfaces in the SLA group showed honeycomb irregular pore structures with different sizes and depths, porous micro-nano spherical hydroxyapatite(HA) deposits are visible on titanium sheet surfaces in the SLA-Bio group, and needle-like nanoparticles were visible on spheriform surfaces. The cell adhesion rate in SLA-Bio group was significantly higher than that in SLA group at the 1h, 6h and 24h after the inoculation of MC3T3 E1 cells(P<0.05),and the cell morphological factors in the SLA-Bio group were significantly lower than those in the SLA group(P<0.05). The results of cell fluorescence staining showed that the SLA-Bio group was superior to the SLA group for adhesion and extension morphology at each time after inoculations.Conclusion SLA-Bio treatment can form porous micro nano spherical HA deposits on pure titanium surface;SLA-Bio surface is more conducive to the adhesion and extension of MC3T3E1 cells when compared to SLA surface, suppo

关 键 词：SLA-Bio表面处理 MC3T3-E1细胞 细胞黏附 细胞形态因子 

分 类 号：R781.05[医药卫生—口腔医学]