产微球茎属(Microbulbifer sp.)FG4菌株琼胶酶功能基因的克隆与表达  

Cloning and expression of a novel gene encoding agarase from Microbulbifer sp.FG4

作　　者：张力雄 刘明明 陈良 方再光[1] Lixiong Zhang;Mingming Liu;Liang Chen;Zaiguang Fang(Ocean College,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学海洋学院,海南海口570228

出　　处：《日用化学工业（中英文）》2023年第3期285-291,共7页China Surfactant Detergent & Cosmetics

基　　金：国家自然科学基金资助项目(40666001)。

摘　　要：以FG4菌株琼胶酶基因侧翼序列为基础,构建重组质粒与载体,从而获得高效产琼胶酶的表达菌株。本研究利用染色体步移法获得FG4菌珠琼胶酶基因功能的侧翼序列之后,将基因组端序列克隆基因片段与表达载体进行重组,并转入到受体细胞BL21中,在鉴定表达菌株的成功构建后利用IPTG诱导表达琼胶酶。实现产微球茎属(Microbulbifer sp.)FG4菌株琼胶酶功能基因的克隆与表达,以期为琼胶酶类在不同领域的实际应用提供理论支持。Based on the flanking sequence of the agarase gene of FG4 strain,the recombinant plasmid and vector were constructed to obtain the engineering strain which could express the agarase efficiently.In this study,the flanking sequence of FG4 agarase gene is obtained by chromosome walking.The cloned gene is recombined with the expression vector pET-22b(+) and transferred into the recipient BL21.After the successful construction of the expressed strain,the expression of agarase is induced by IPTG and the optimal fermentation conditions of the expressed strain are explored.This strain ensures the catalytic activity of agarase and lays the foundation for the agarasebased pharmaceutical industry,daily chemical industry and food industry.The cloning and expression of agarase gene from Microbulbifers sp.FG4 strain of Microbulbifers provides theoretical support for agarase application in different fields.

关 键 词：产微球茎属 琼胶酶 染色体步移 克隆与表达 

分 类 号：TQ658[化学工程—精细化工]