高效液相色谱法测定猪血制品中苋菜红、新红、胭脂红和诱惑红  

Determination of Natural Amaranthus Red, New Red,Carmine and Allura Red in Pig Blood Products by HPLC

作　　者：毕娜 高晗 杨晓赫 BI Na;GAO Han;YANG Xiaohe(Jilin Food and Drug Inspection Institute,Jilin 132000,China)

机构地区：[1]吉林市食品药品检验所,吉林吉林132000

出　　处：《食品安全导刊》2022年第33期106-109,共4页China Food Safety Magazine

摘　　要：目的:建立同时测定猪血制品中苋菜红、新红、胭脂红、诱惑红4种色素的方法。方法:采用无水乙醇-氨水-水(7∶2∶1)提取,经旋涡振荡、超声、离心、浓缩、定容,用高效液相色谱法测定,二极管阵列为检测器,色谱柱为C18柱(250 mm×4.6mm,5μm),流动相为0.02 mol·L^(-1)乙酸铵-甲醇,梯度洗脱,检测波长为510 nm。结果:苋菜红、新红、胭脂红、诱惑红在0.1~100.0μg·mL^(-1)线性关系良好,相关系数均为1.000 0。检出限均为0.05 mg·kg-1,平均加标回收率为81%~102%。结论:本方法灵敏度高,分离效果好,结果稳定可靠,适用于猪血产品中苋菜红、新红、胭脂红、诱惑红的测定。Objective: To establish a method for simultaneous determination of amaranth, new red, carmine red and temptation red in pig blood products. Method: Aqueous ethanol-ammonium-water(7 ∶ 2 ∶ 1) extraction was performed by vortex oscillation, ultrasound, centrifugation, concentration and constant volume. The determination was performed by high performance liquid chromatography(HPLC) with diode array as detector. The determination was performed on C18column(250 mm×4.6 mm, 5 μm) with 0.02 mol·L^(-1)ammonium acetate-methanol gradient elution as mobile phase. The detection wavelength was 510 nm. Result: Amaranth, new red, carmine red and temptation red had good linearity in 0.1 ~ 100.0 μg·mL^(-1), and the correlation coefficients were all 1.000 0. The limits of detection were 0.05 mg·kg-1, the average recoveries were 81% ~ 102%. Conclusion: The method has high sensitivity, good separation effect, stable and reliable results, and is suitable for the determination of amaranth red,new red, carmine red and temptation red in pig blood products.

关 键 词：猪血制品 苋菜红 新红 胭脂红 诱惑红 高效液相色谱 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]