库布齐沙漠生物土壤结皮细菌群落结构及多样性  

Community Structure and Diversity of Bacterial in Biological Soil Crusts of Hobq Desert

作　　者：刘雪锋[1,2] 刘矜杰 陈晓锋 梁海荣 刘平生[1,2] 莎仁图雅 LIU Xue-feng;LIU Jin-jie;CHEN Xiao-feng;LIANG Hai-rong;LIU Ping-sheng;Sharentuya(Inner Mongolia Academy of Forestry Science,Hohhot 010010,China;Key Laboratory of Desert Ecosystem and Ecological Engineering of Inner Mongolia Autonomous Region,Hohhot 010010,China;Inner Mongolia Lvtong Environmental Greening Limited Company,Hohhot 010010,China;West Ujimqin Banner Forestry Development Center,Xilin Gol League,Inner Mongolia,Xilin Gol 026200,China)

机构地区：[1]内蒙古自治区林业科学研究院,内蒙古呼和浩特010010 [2]内蒙古自治区沙地(沙漠)生态系统与生态工程重点实验室,内蒙古呼和浩特010010 [3]内蒙古绿通环境绿化科技有限责任公司,内蒙古呼和浩特010010 [4]西乌珠穆沁旗林业事业发展中心,内蒙古锡林郭勒026200

出　　处：《内蒙古林业科技》2022年第4期21-27,共7页Journal of Inner Mongolia Forestry Science and Technology

基　　金：内蒙古自然科学基金(2019MS03007);国家“十三五”项目(2016YFC050090403)。

摘　　要：为探明库布齐沙漠不同演替阶段生物土壤结皮(BSCs)细菌群落结构及其与土壤化学性质关系,本研究借助Ion S5TMXL测序平台对库布齐沙漠不同区域BSCs细菌的16S rRNA基因进行测序,对比分析了各采样点裸沙、藻结皮和苔藓结皮细菌的群落结构、多样性及其与土壤化学性质之间的关系。结果表明:(1)随着BSCs发育演替,土壤有机质(OM)、总氮(TN)、总叶绿素a(Chla)、总糖(TS)呈增加趋势,且OM和TS最为显著,而pH值呈降低趋势;(2)和裸沙比较,藻结皮中,蓝藻门占比由3.11%上升为22.88%,酸杆菌门占比从3.53%上升为7.88%,而苔藓结皮中,蓝藻门占比降低到8.78%,酸杆菌门占比升高到12.21%;(3)苔藓结皮与OM、总磷(TP)、TN、TS、Chla呈正相关,而与pH值呈负相关,OM和pH值是影响裸沙群落组成的主要因素。To investigate the bacterial community structure of biological soil crusts(BSCs) in different stages of succession and their relationships with soil chemical properties in Hobq Desert, in this study, 16SrRNA genes of BSCs from different areas in Hobq Desert were sequenced with the help of Ion S5TM XL sequencing platform, the community structure and diversity of bare sand, algal crusts and moss crusts bacteria and their relationships with soil chemical properties at each sampling site were compared and analyzed. The results showed that:(1)With the development and succession of BSCs, the contents of organic matter(OM), total nitrogen(TN), total chlorophyll(chla) and total sugar(TS) tended to increase, OM and TS were the most significant, while pH tended to decrease.(2) Compared with bare sand, the percentage of Cyanobacteria increased from 3.11% to 22.88% and the one of Acidobacteria increased from 3.53% to 7.88% in algal crusts, while the percentage of Cyanobacteria decreased to 8.78% and the one of Acidobacteria increased to 12.21% in moss crusts.(3) Moss crusts were positively correlated with OM, total phosphorus(TP), TN, TS and Chla, and negatively correlated with pH. OM and pH were the main factors affecting the bacterial community structure.

关 键 词：库布齐沙漠 BSCS 高通量测序 土壤细菌 群落结构 

分 类 号：S154[农业科学—土壤学]