西南典型种植区蔬菜重金属健康风险评估  

Health Risk Assessment of Heavy Metals in Vegetables from Typical Planting Areas in Southwest of China

作　　者：田稳 汪洁 岳志强 马娇阳 李梦莹[1] 王健敏[3] 徐武美 向萍 Tian Wen;Wang Jie;Yue Zhiqiang;Ma Jiaoyang;Li Mengying;Wang Jianmin;Xu Wumei;Xiang Ping(School of Ecology and Environment,Southwest Forestry University,Kunming 650224;Agricultural Environmental Protection and Rural Energy Workstation of Yuxi,Yuxi 653199,Yunnan;Yunnan Rural Science and Technology Service Centre,Kunming 650021;School of Energy and Environment Science,Yunnan Normal University,Kunming 650500)

机构地区：[1]西南林业大学生态与环境学院,昆明650224 [2]玉溪市农业环境保护和农村能源工作站,云南玉溪653199 [3]云南省农村科技服务中心,昆明650021 [4]云南师范大学能源与环境科学学院,昆明650500

出　　处：《中国食品学报》2023年第2期319-329,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：云南省农业联合专项重点项目(202101BD 070001-023);国家自然科学基金项目(41967026);国家林业和草原局林草科技创新青年拔尖人才项目(2020132613);云南省高层次人才引进计划青年人才项目(YNQR-QNRC-2018-049);云南省创新团队项目(202005AE160017)。

摘　　要：以玉溪市红塔区、通海县和元江县土壤-蔬菜系统为研究对象,分析土壤和蔬菜中Cd、As、Pb、Cr的积累特征,结合体外胃肠模拟法和肠道细胞Caco-2毒性评估蔬菜中重金属生物可给性与人体健康风险。结果表明:通海和红塔菜地土壤受As、Cd或Pb污染,其芸薹类蔬菜中Cd的含量分别超出标准限量值13.6倍和2.2倍。通海西兰花、白菜中Cd在胃阶段生物可给性分别为36.40%和9.84%,肠阶段分别下降到0.91%和0%,元江鱼腥草中As在胃、肠阶段的生物可给性分别为25.23%和6.69%。基于重金属总含量的评估,通海芸薹类蔬菜存在潜在健康风险,而基于生物可给性的评价,以上蔬菜均不存在健康风险。细胞毒性试验表明生物可给态重金属暴露后不会抑制细胞活性,验证了基于生物可给性健康风险评估更为准确。结论:重金属生物可给性分析结合肠道细胞毒性的风险评估方法,可更准确评价蔬菜中重金属健康风险。In this study,the accumulation characteristics and health risks of heavy metals Cd,As,Pb and Cr in soil-vegetable system of Hongta District(HT),Tonghai County(TH)and Yuanjiang County(YJ)in Yuxi city were analyzed.In addition,some vegetables with high concentrations of heavy metal were screened and their cytotoxicity was investigated by in vitro gastrointestinal simulation method(SBRC)and intestinal cell Caco-2 model.The results showed that TH and HT soils were contaminated by As,Cd and/or Pb.The content of Cd in TH and HT brassica vegetables exceeded the limit value of GB 2762-2017 by 13.6 folds and 2.2 folds.The bioaccessibility of TH Brassica oleracea L.var.botrytis L.and Brassica pekinensis(Lour.)Rupr.in gastric stage was 36.40% and 9.84%,and decreased to 0.91% and 0% in intestinal stage,respectively.The bioaccessibility of YJ Houttuynia cordata Thunb.in gastric stage and intestinal stage were 25.23% and 6.69%,respectively.The health risk assessment based on the total content of heavy metals showed TH brassica vegetable may had potential health risks,while bioaccessibility date showed that there was no health risk for the three vegetables.To validate the above-mentioned results,Caco-2 cells were exposed to all bioaccessible extracts of vegetables,and cell viability was not significantly decreased at 4 h and 8 h in all groups.Collectively,based on the total of heavy metals in vegetables may overestimate their health risk,while the new assessment method based on bioaccessibility data and cytotoxicity maybe a more accurate approach for health risk assessment.

关 键 词：蔬菜 重金属 健康风险 CACO-2细胞 

分 类 号：X56[环境科学与工程—环境工程] X826